Surgical Bioengineering Laboratory, Department of Surgery, University of California, Davis School of Medicine, Sacramento, CA 95817, United States.
Surgical Bioengineering Laboratory, Department of Surgery, University of California, Davis School of Medicine, Sacramento, CA 95817, United States.
Placenta. 2017 Nov;59:163-168. doi: 10.1016/j.placenta.2017.03.022. Epub 2017 Apr 2.
Determine the effect of 2-octylcyanoacrylate on placenta derived mesenchymal stromal cells (PMSCs) seeded onto extracellular matrix (ECM) in order to assess its biocompatibility as a potential adhesive for in-vivo fetal cell delivery.
PMSCs isolated from chorionic villus tissue were seeded onto ECM. A MTS proliferation assay assessed cellular metabolic activity at various time points in PMSC-ECM with direct, indirect, and no glue contact. Conditioned media collected prior to and 24 hours after glue exposure was analyzed for secretion of human brain-derived neurotrophic factor, hepatocyte growth factor, and vascular endothelial growth factor.
Direct and indirect contact with 2-octylcyanoacrylate results in progressively decreased cellular metabolic activity over 24 hours compared to no glue controls. Cells with direct contact are less metabolically active than cells with indirect contact. 24 hours of glue exposure resulted in suppression of growth factor secretion that is near complete with direct contact.
Exposure to 2-octylcyanoacrylate results in decreased metabolic activity and decreased measurable secretion of growth factors by PMSCs seeded onto ECM. Thus, the application of 2-octylcyanoacrylate glue should be limited when working with cell-engineered scaffolds as its inhibitory effects on cell growth and secretory function can limit the therapeutic potential of cell-based interventions.
确定 2-辛基氰基丙烯酸酯对细胞外基质（ECM）上种植的胎盘间充质基质细胞（PMSC）的影响，以评估其作为体内胎儿细胞递送潜在粘合剂的生物相容性。
从绒毛膜绒毛组织中分离出 PMSC 并种植在 ECM 上。MTS 增殖测定法在 PMSC-ECM 中直接、间接和无胶接触的不同时间点评估细胞代谢活性。在涂胶前和涂胶后 24 小时收集条件培养基，分析人脑源性神经营养因子、肝细胞生长因子和血管内皮生长因子的分泌情况。
与无胶对照相比，直接和间接接触 2-辛基氰基丙烯酸酯会导致细胞代谢活性在 24 小时内逐渐降低。直接接触的细胞比间接接触的细胞代谢活性更低。24 小时的胶暴露导致生长因子分泌受到抑制，直接接触时几乎完全抑制。
暴露于 2-辛基氰基丙烯酸酯会导致种植在 ECM 上的 PMSC 的代谢活性降低和可测量的生长因子分泌减少。因此，在使用细胞工程支架时，应限制 2-辛基氰基丙烯酸酯胶的应用，因为其对细胞生长和分泌功能的抑制作用会限制基于细胞的干预措施的治疗潜力。
