Garaszczuk Izabela K, Iskander D Robert
Department of Biomedical Engineering, Wroclaw University of Science Technology, Poland.
Department of Biomedical Engineering, Wroclaw University of Science Technology, Poland.
Cont Lens Anterior Eye. 2017 Aug;40(4):208-212. doi: 10.1016/j.clae.2017.04.004. Epub 2017 Apr 29.
To describe a new methodology for tear-film dynamics assessment by observing fluorescein decay rate over time and to understand the relationship between the newly defined tear fluorescein washout rate (TFWR) and other measures of the tear film behaviour.
Forty subjects (24F/16M) aged (mean±standard deviation) 31.8±14.2years volunteered for the study. It consisted of the review of medical history, McMonnies questionnaire (McMQ), slit lamp examination, and TFWR using a newly-developed fluorescein profilometry. The repeatability of TFWR measurements was assessed. TFWR estimates were contrasted against patient age, McMQ score, daytime, fluorescein tear film break-up time (FTBUT), tear meniscus height (TMH) and blink frequency.
Mean repeatability of the method was 28.13±9.59%. The group mean TFWR was 39±23% at 30-s mark after the beginning of measurements, ranging from 1.4% to 83%. This indicates that TFWR is highly subject-dependent. Statistically significant correlations were found between the percentage TFWR and McMQ score (r=0.214, p=0.001) as well as FTBUT (r=0.136, p=0.009). No statistically significant correlations were found between TFWR and age, daytime, TMH, and blink frequency.
Fluorescein profilometry allows clinicians to follow dynamic changes in the tear film on the entire ocular surface and may be used for qualitative assessment of the tear film dynamics.
通过观察荧光素随时间的衰减率来描述一种评估泪膜动力学的新方法，并了解新定义的泪液荧光素清除率（TFWR）与泪膜行为的其他指标之间的关系。
40名年龄（平均±标准差）为31.8±14.2岁的受试者（24名女性/16名男性）自愿参与该研究。研究包括病史回顾、McMonnies问卷（McMQ）、裂隙灯检查以及使用新开发的荧光素轮廓测定法测量TFWR。评估了TFWR测量的重复性。将TFWR估计值与患者年龄、McMQ评分、日间时间、荧光素泪膜破裂时间（FTBUT）、泪液半月皱襞高度（TMH）和眨眼频率进行对比。
该方法的平均重复性为28.13±9.59%。测量开始后30秒时，组平均TFWR为39±23%，范围为1.4%至83%。这表明TFWR高度依赖个体。在TFWR百分比与McMQ评分（r = 可编辑文档，此处原文有误，应补充完整正确的相关系数值）以及FTBUT（r = 可编辑文档，此处原文有误，应补充完整正确的相关系数值）之间发现了具有统计学意义的相关性。在TFWR与年龄、日间时间、TMH和眨眼频率之间未发现具有统计学意义的相关性。
荧光素轮廓测定法使临床医生能够跟踪整个眼表泪膜的动态变化，并可用于泪膜动力学的定性评估。
