MicroRNA-497 suppresses renal cell carcinoma by targeting VEGFR-2 in ACHN cells.

Abnormal expression of miRNAs contributed to cancers through regulation of proliferation, apoptosis and drug resistance of cancer cells. The present study was designed to investigate the effect of on renal cell carcinoma (RCC) and its possible mechanism. Forty paired clear cell RCC (ccRCC) tissues and adjacent normal kidney tissues were obtained from patients, who were not treated by chemotherapy or radiotherapy. RT-PCR was performed to detect expression of in the ccRCC tissues. Effects of on cell viability, apoptosis, migration and invasion were detected in ACHN cells. Western blotting (WB) was employed to detect the downstream targets of We found that in ccRCC tissues was decreased. We treated ACHN cells with mimics and inhibitors and found that inhibited viability, migration and invasion of ACHN cells. promoted ACHN cells' apoptosis. VEGFR-2 was predicted as a possible target of Luciferase reporter assay proved that suppressed VEGFR-2 directly by binding to its 3'-UTR. Further studies showed that influenced the MEK/ERK and p38 MAPK signalling pathways. Our findings demonstrated that could suppress RCC by targeting VEGFR-2.