INSERM UMR-S1147, CNRS SNC5014, Paris Descartes University, Equipe labellisée Ligue Nationale contre le cancer, Paris, France.
CNRS, Univ. Bordeaux, CRPP, UPR 8641, 115 Avenue Schweitzer, 33600, Pessac, France.
Sci Rep. 2017 May 2;7(1):1366. doi: 10.1038/s41598-017-01454-4.
Droplet-based microfluidics is extensively and increasingly used for high-throughput single-cell studies. However, the accuracy of the cell counting method directly impacts the robustness of such studies. We describe here a simple and precise method to accurately count a large number of adherent and non-adherent human cells as well as bacteria. Our microfluidic hemocytometer provides statistically relevant data on large populations of cells at a high-throughput, used to characterize cell encapsulation and cell viability during incubation in droplets.
基于液滴的微流控技术被广泛且日益地用于高通量单细胞研究。然而，细胞计数方法的准确性直接影响到此类研究的稳健性。在这里，我们描述了一种简单而精确的方法，可准确计数大量贴壁和非贴壁的人类细胞以及细菌。我们的微流控血球计数板可提供高通量下大量细胞群体的统计学上相关的数据，用于在液滴中孵育期间对细胞包封和细胞活力进行特征描述。
