车前草花叶病毒一个不同分离株在……中的分子检测与特性分析
Molecular detection and characterization of a divergent isolate of Plantago asiatica mosaic virus in .
作者信息
Lim Seungmo, Igori Davaajargal, Zhao Fumei, Do Yun Su, Cho In-Sook, Choi Gug-Seoun, Moon Jae Sun
机构信息
Molecular Biofarming Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141 Republic of Korea.
Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, 34113 Republic of Korea.
出版信息
Virusdisease. 2016 Sep;27(3):307-310. doi: 10.1007/s13337-016-0329-5. Epub 2016 Jul 21.
Four samples of showing mottle or mottled yellowing symptoms were collected and three of them were confirmed to be infected with Plantago asiatica mosaic virus (PlAMV) by Illumina HiSeq 2500 paired-end RNA sequencing. Consequently, the complete genomic sequence of a Korean isolate of PlAMV (PlAMV isolate Gunwi) from was determined. The nucleotide sequence of PlAMV-Gunwi was most closely related to a Russian isolate of PlAMV (PlAMV-Ru) collected from but highly dissimilar (~23 %) to other isolates of PlAMV from other plant species. Pairwise comparisons revealed that the complete replicase protein and coat protein of PlAMV-Gunwi share 85.15-91.20 and 84.54-90.82 % amino acid sequence identities, respectively, to the corresponding proteins of other PlAMV isolates. To our knowledge, this is the first report of the natural infection of by PlAMV in Korea, and the divergent PlAMV isolate Gunwi expands our understanding of the epidemiology of PlAMV.
采集了4份表现出斑驳或斑驳黄化症状的样本，其中3份通过Illumina HiSeq 2500双末端RNA测序被确认为感染了车前草花叶病毒（PlAMV）。因此，确定了来自韩国的PlAMV分离株（PlAMV株系Gunwi）的完整基因组序列。PlAMV-Gunwi的核苷酸序列与从采集的俄罗斯PlAMV分离株（PlAMV-Ru）关系最为密切，但与来自其他植物物种的其他PlAMV分离株差异很大（约23%）。成对比较显示，PlAMV-Gunwi的完整复制酶蛋白和外壳蛋白与其他PlAMV分离株的相应蛋白的氨基酸序列同一性分别为85.15-91.20%和84.54-90.82%。据我们所知，这是韩国首次报道PlAMV对的自然感染，并且不同的PlAMV分离株Gunwi扩展了我们对PlAMV流行病学的理解。
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