Structural Revision and Biosynthesis of the Fungal Phytotoxins Phyllostictines A and B.

The structure of the fungal phytotoxins known as the phyllostictines has been revised to a series of bicyclic 3-methylene tetramic acids. Genome sequencing of the producing organism Phyllostica cirsii has revealed a biosynthetic gene cluster responsible for the biosynthesis of the phyllostictines, and targeted knockout experiments have proven the link and produced an intermediate.