Ferrante Jason A, Cortés-Hinojosa Galaxia, Archer Linda L, Wellehan James F X
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL.
J Vet Diagn Invest. 2017 Jul;29(4):476-482. doi: 10.1177/1040638717707554. Epub 2017 May 3.
Trichechid herpesvirus 1 (TrHV-1) is currently the only known herpesvirus in any sirenian. We hypothesized that stress may lead to recrudescence of TrHV-1 in manatees, thus making TrHV-1 a potential biomarker of stress. We optimized and validated a TrHV-1 real-time quantitative probe hybridization PCR (qPCR) assay that was used to quantify TrHV-1 in manatee peripheral blood mononuclear cells (PBMCs). Average baseline TrHV-1 loads in a clinically healthy wild Florida manatee ( Trichechus manatus latirostris) population ( n = 42) were 40.9 ± SD 21.2 copies/100 ng DNA; 19 of 42 manatees were positive. TrHV-1 loads were significantly different between the 2 field seasons ( p < 0.025). This optimized and validated qPCR assay may be used as a tool for further research into TrHV-1 in Florida manatees.
海牛疱疹病毒1型（TrHV-1）是目前已知的唯一一种存在于海牛目动物中的疱疹病毒。我们推测，应激可能会导致海牛体内的TrHV-1复发，从而使TrHV-1成为一种潜在的应激生物标志物。我们优化并验证了一种TrHV-1实时定量探针杂交PCR（qPCR）检测方法，该方法用于定量海牛外周血单个核细胞（PBMC）中的TrHV-1。在临床健康的佛罗里达野生海牛（Trichechus manatus latirostris）种群（n = 42）中，TrHV-1的平均基线载量为40.9±标准差21.2拷贝/100 ng DNA；42头海牛中有19头呈阳性。两个野外季节之间的TrHV-1载量存在显著差异（p < 0.025）。这种经过优化和验证的qPCR检测方法可作为进一步研究佛罗里达海牛中TrHV-1的工具。
