Rosenberg J, Pines M, Hurwitz S
Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.
J Endocrinol. 1988 Sep;118(3):447-53. doi: 10.1677/joe.0.1180447.
Dispersed chick adrenocortical cells were incubated with mammalian and avian angiotensin-II, Ca2+, K+, verapamil, nifedipine, Ca2+ ionophore (A23187), protein kinase-C activator (phorbol 12-myristate 13-acetate; TPA), atrial natriuretic peptide (ANP), sodium nitroprusside (SNP) and ACTH. Secretion of aldosterone and corticosterone, and accumulation of cyclic nucleotides were assessed. Secretion of aldosterone was not affected by angiotensin-II, Ca2+ channel blockers, Ca2+ ionophore or TPA. ANP stimulated production of cyclic GMP (cGMP), and inhibited aldosterone secretion with a similar dose-response relationship. SNP also stimulated cGMP production and inhibited the ACTH-stimulated aldosterone secretion. The results indicate that ANP is an inhibitor of aldosterone secretion in birds and suggest that this inhibition is mediated by cGMP. In contrast to mammalian glomerulosa cells, angiotensin-II and the calcium-inositol phosphate-protein kinase C pathway appear not to be involved in the regulation of aldosterone secretion by avian adrenal cells.
将分散的鸡肾上腺皮质细胞与哺乳动物和禽类的血管紧张素 II、钙离子、钾离子、维拉帕米、硝苯地平、钙离子载体（A23187）、蛋白激酶 C 激活剂（佛波醇 12 - 肉豆蔻酸酯 13 - 乙酸酯；TPA）、心房利钠肽（ANP）、硝普钠（SNP）和促肾上腺皮质激素（ACTH）一起孵育。评估醛固酮和皮质酮的分泌以及环核苷酸的积累。血管紧张素 II、钙离子通道阻滞剂、钙离子载体或 TPA 均不影响醛固酮的分泌。ANP 刺激环鸟苷酸（cGMP）的产生，并以类似的剂量反应关系抑制醛固酮分泌。SNP 也刺激 cGMP 的产生，并抑制 ACTH 刺激的醛固酮分泌。结果表明，ANP 是鸟类醛固酮分泌的抑制剂，并提示这种抑制作用是由 cGMP 介导的。与哺乳动物的球状带细胞不同，血管紧张素 II 和钙 - 肌醇磷酸 - 蛋白激酶 C 途径似乎不参与禽类肾上腺细胞醛固酮分泌的调节。
