Weniger J P, Zeis A
Laboratoire de Zoologie et d'Embryologie expérimentale, Université Louis Pasteur, Strasbourg, France.
J Endocrinol. 1988 Sep;118(3):485-9. doi: 10.1677/joe.0.1180485.
The effect of dibutyryl cyclic AMP and FSH on oestrogen biosynthesis was investigated in testes from 18- to 21-day-old fetal rats cultured in vitro in the presence of tritiated testosterone. Oestrone and oestradiol concentrations were measured by determination of constant specific activity after isotopic dilution. Dibutyryl cyclic AMP and FSH markedly stimulated the conversion of testosterone into both oestrone and oestradiol at all stages studied. Oestradiol synthesis was stimulated by two- to sevenfold, while stimulation of oestrone synthesis was even greater. The results demonstrate that the aromatase enzyme system of the fetal rat testis responds to cyclic AMP and FSH.
在存在氚化睾酮的情况下，对18至21日龄胎鼠的睾丸进行体外培养，研究了二丁酰环磷腺苷（dibutyryl cyclic AMP）和促卵泡激素（FSH）对雌激素生物合成的影响。通过同位素稀释后测定恒定比活性来测量雌酮和雌二醇的浓度。在所有研究阶段，二丁酰环磷腺苷和促卵泡激素均显著刺激睾酮向雌酮和雌二醇的转化。雌二醇的合成受到2至7倍的刺激，而对雌酮合成的刺激更大。结果表明，胎鼠睾丸的芳香化酶系统对环磷腺苷和促卵泡激素有反应。
