Weniger J P
Laboratoire de Zoologie et d'Embryologie expérimentale de l'Université Louis-Pasteur, Strasbourg, France.
J Steroid Biochem Mol Biol. 1993 Mar;44(4-6):459-62. doi: 10.1016/0960-0760(93)90250-z.
Aromatase activity in fetal rat testes and ovaries was demonstrated by the conversion of tritiated testosterone or 19-hydroxyandrostenedione into estrone and estradiol, which were identified and quantified by double isotopic dilution and recrystallization to constant specific activity. Testes formed mostly estradiol, ovaries mostly estrone. Aromatase activity was stimulated by cAMP in both the testes and ovaries as early as 17 days of fetal life. Stimulation by FSH was noted at this same stage in the testis, but not before 3-4 days after birth in the ovary. LH was without effect on aromatase activity in both kinds of gonads. Basal estrogen secretion was non-existent or undetectable in both the testes and ovaries in fetal stages. In the presence of cAMP and as early as 17 days of fetal life, the testes released estradiol, as early as 14 days the ovaries released estrone. Estrogen secretion was stimulated by LH and FSH at fetal stages in the testis and at infantile stages in the ovary. Responsiveness to gonadotrophins closely followed the appearance of the receptors.
通过将氚标记的睾酮或19-羟基雄烯二酮转化为雌酮和雌二醇，证明了胎鼠睾丸和卵巢中的芳香化酶活性，通过双同位素稀释和重结晶至恒定比活性来鉴定和定量。睾丸主要生成雌二醇，卵巢主要生成雌酮。早在胎儿期17天时，cAMP就能刺激睾丸和卵巢中的芳香化酶活性。在睾丸中，同一阶段可观察到FSH的刺激作用，但在卵巢中，出生后3 - 4天之前未观察到。LH对两种性腺中的芳香化酶活性均无影响。在胎儿期，睾丸和卵巢中均不存在或检测不到基础雌激素分泌。在cAMP存在的情况下，早在胎儿期17天时，睾丸就释放雌二醇，早在14天时，卵巢就释放雌酮。在胎儿期，LH和FSH刺激睾丸分泌雌激素，在婴儿期，刺激卵巢分泌雌激素。对促性腺激素的反应性与受体的出现密切相关。
