Fenwick M L, Owen S A
Dunn School of Pathology, Oxford University, U.K.
J Gen Virol. 1988 Nov;69 ( Pt 11):2869-77. doi: 10.1099/0022-1317-69-11-2869.
The alpha or immediate early mRNA of herpes simplex virus strain HSV-2(G) had a half-life of about 15 min if made in the absence of viral protein synthesis but was relatively stable if viral protein synthesis occurred, either freely or restricted by the presence of the proline analogue azetidine. In contrast, the alpha mRNA of other strains of the virus is stable, even in the absence of protein synthesis. Studies with recombinant viruses showed that the region of the viral DNA between 0.58 and 0.65 map units [which includes the gene (vhs, UL41) that controls virion-mediated shutoff of host protein synthesis] is important in determining the survival of alpha mRNA. In mixed infection experiments HSV-2(G) inhibited alpha as well as host protein synthesis but the shutoff activity appeared to be short-lived. Within 3 h after infection, as a result of protein synthesis, cells became completely resistant to shutoff by superinfecting virus.
单纯疱疹病毒2型（G）株的α或即刻早期mRNA，若在无病毒蛋白合成的情况下产生，其半衰期约为15分钟，但如果发生病毒蛋白合成，无论是自由合成还是受脯氨酸类似物氮杂环丁烷存在的限制，它都相对稳定。相比之下，该病毒其他毒株的α mRNA即使在无蛋白合成的情况下也是稳定的。对重组病毒的研究表明，病毒DNA中位于0.58至0.65图谱单位之间的区域[其中包括控制病毒体介导的宿主蛋白合成关闭的基因（vhs，UL41）]在决定α mRNA的存活方面很重要。在混合感染实验中，HSV-2（G）抑制α以及宿主蛋白合成，但关闭活性似乎是短暂的。感染后3小时内，由于蛋白合成，细胞对超感染病毒的关闭作用完全产生抗性。
