Mutlu Oguz, Ulak Guner, Akar Furuzan, Erden Faruk, Celikyurt Ipek Komsuoglu, Bektas Emine, Tanyeri Pelin, Kaya Havva
Kocaeli University Medical Faculty, Department of Pharmacology, 41380, Kocaeli, Turkey.
Sakarya University, Faculty of Medicine, Department of Pharmacology, 54100, Sakarya, Turkey.
Chin J Physiol. 2017 Apr 30;60(2):106-113. doi: 10.4077/CJP.2017.BAF450.
The neurosecretory cells in the corpus cardiacum of insects synthesize a set of hormones that are called adipokinetic, hypertrehalosemic or hyperprolinemic depending on the insect in question. They are the Adipokinetic Hormone/Red Pigment-Concentrating Hormone (AKH/RPCH) family of peptides. The present study investigated the effects of acute administration of Locusta Migratoria (Locmi-AKHII) and Anax Imperator (Anaim-AKH) on depression, anxiety, pain (analgesy), locomotion and memory in mice in forced swimming (FST), elevated plus maze (EPM), hot plate, locomotor activity and passive avoidance tests. Both Locmi-AKH-II (4 mg/kg) and Anaim-AKH (0.25 and 0.50 mg/kg) decreased immobility time (in sec, s) in the FST test. Anaim-AKH (0.5 and 1 mg/kg) increased the percentage of time spent in open arms/total time spent and the percentage of the number of open arm/total arm entries in the EPM test. Anaim-AKH (1 and 2 mg/kg) significantly increased latency (s) (initial time passed) for mice to lick their hind paws or jumping in the hot plate test. Anaim-AKH (4 mg/kg) significantly decreased the total distance (cm) moved, or the speed (cm/s) of movement of the animals in the locomotor activity test. Neither Locmi-AKH-II nor Anaim-AKH altered the retention latency (s) in the passive avoidance test. Both Locmi-AKH-II and Anaim-AKH exerted antidepressant effects, while only Anaim-AKH had anxiolytic and analgesic effects when administered acutely. Anaim-AKH diminished locomotion at higher doses while Locmi-AKH-II had no such effects. Neither Locmi-AKH-II nor Anaim-AKH disturbed learning and memory when acutely administered. Data of our studies suggest clinical potentials of AKH to be used in depression, anxiety and pain without disturbing memory.
昆虫心侧体中的神经分泌细胞会合成一组激素，根据所涉及的昆虫种类不同，这些激素被称为脂肪动激素、高海藻糖血激素或高脯氨酸血激素。它们属于脂肪动激素/红色素浓缩激素（AKH/RPCH）肽家族。本研究在强迫游泳试验（FST）、高架十字迷宫试验（EPM）、热板试验、自发活动试验和被动回避试验中，探究了急性给予飞蝗（Locmi - AKHII）和大蜻蜓（Anaim - AKH）对小鼠抑郁、焦虑、疼痛（镇痛）、运动和记忆的影响。在FST试验中，Locmi - AKH - II（4毫克/千克）和Anaim - AKH（0.25毫克/千克和0.50毫克/千克）均减少了不动时间（以秒计，s）。在EPM试验中，Anaim - AKH（0.5毫克/千克和1毫克/千克）增加了在开放臂停留时间占总时间的百分比以及进入开放臂次数占总臂次数的百分比。在热板试验中，Anaim - AKH（1毫克/千克和2毫克/千克）显著增加了小鼠舔后爪或跳跃的潜伏期（s）（初始经过时间）。在自发活动试验中，Anaim - AKH（4毫克/千克）显著降低了动物移动的总距离（厘米）或移动速度（厘米/秒）。Locmi - AKH - II和Anaim - AKH均未改变被动回避试验中的记忆潜伏期（s）。Locmi - AKH - II和Anaim - AKH急性给药时均具有抗抑郁作用，而只有Anaim - AKH具有抗焦虑和镇痛作用。高剂量时Anaim - AKH会减少运动，而Locmi - AKH - II无此作用。Locmi - AKH - II和Anaim - AKH急性给药时均不会干扰学习和记忆。我们的研究数据表明，AKH在用于治疗抑郁、焦虑和疼痛且不干扰记忆方面具有临床应用潜力。
