Institute of Entomology, Biology Centre ASCR, Ceské Budejovice, Czech Republic.
Comp Biochem Physiol B Biochem Mol Biol. 2010 May;156(1):26-32. doi: 10.1016/j.cbpb.2010.01.015. Epub 2010 Feb 6.
The diacylglycerols (DG) molecular species and their fatty acid (FA) composition were investigated by electrospray mass spectrometry (ESI-MS) and by gas chromatography with flame ionisation detection (GC-FID) in haemolymph of Locusta migratoria after application of adipokinetic hormones Locmi-AKH-I, -II and -III. The analyses showed (1) a heterogeneous distribution of individual DGs in haemolymph after the hormone application. The results revealed that mobilization of the DGs is molecular species-specific with the highest proportion of 34:1 DG (16:0/18:1 - mw 594Da) for all Locmi-AKHs bearing palmitic acid (C16:0) and oleic acid (C18:1) residues, and forming about 20% of the total mobilized DG content. (2) Analysis of fat body triacylglycerols revealed that all Locmi-AKHs mobilize the DGs selectively with the preference of those possessing the C18 and C16 FAs. The fat body FAs with carbon chain longer than 18 did not participate in the mobilization. (3) A distribution of FAs in the DG structures obtained by LC/ESI-MS, and FA analysis by GC-FID after transmethylation indicated a certain degree of Locmi-AKH selectivity toward the mobilized DGs and hence the FAs. The Locmi-AKH-I significantly prefers mobilization of DGs containing unsaturated FAs, while Locmi-AKH-II and -III prefer mobilization of saturated FAs.
应用昆虫激素Locmi-AKH-I、-II 和 -III 后，通过电喷雾质谱 (ESI-MS) 和火焰离子化检测气相色谱 (GC-FID) 研究了 Locusta migratoria 血淋巴中的二酰基甘油 (DG) 分子种类及其脂肪酸 (FA) 组成。分析表明：（1）激素作用后血淋巴中单个 DG 呈异质分布。结果表明，DG 的动员具有分子种类特异性，具有棕榈酸 (C16:0) 和油酸 (C18:1) 残基的所有 Locmi-AKH 以最高比例的 34:1 DG (16:0/18:1 - mw 594Da) 被动员，占总动员 DG 含量的约 20%。（2）对脂肪体三酰甘油的分析表明，所有 Locmi-AKH 都选择性地动员 DG，优先选择含有 C18 和 C16 FA 的 DG。碳链长于 18 的脂肪体 FA 不参与动员。（3）通过 LC/ESI-MS 获得的 DG 结构中的 FA 分布和 GC-FID 分析经甲酯化后表明，Locmi-AKH 对动员的 DG 及其 FA 具有一定的选择性。Locmi-AKH-I 显著偏爱动员含有不饱和 FA 的 DG，而 Locmi-AKH-II 和 -III 偏爱动员饱和 FA。
