Oliveira Cleide Correia, Oliveira Clarissa Vasconcelos, Grigoletto Jéssica, Ribeiro Leandro Rodrigo, Funck Vinícius Rafael, Meier Letícia, Fighera Michele Rechia, Royes Luiz Fernando Freire, Furian Ana Flávia, Menezes Irwin Rose Alencar, Oliveira Mauro Schneider
a Graduate Program of Biological Sciences: Biochemistry , Federal University of Santa Maria , Santa Maria , Brazil.
b Department of Nursing , Regional University of Cariri , Crato , Brazil.
Neurol Res. 2017 Aug;39(8):667-674. doi: 10.1080/01616412.2017.1324380. Epub 2017 May 3.
Epilepsy is a common brain disease and a major worldwide public health problem. The seizures in a significant number of patients suffering from epilepsy remain inadequately controlled by currently available pharmacological treatments. Accordingly, there is a need for the discovery of new anticonvulsant approaches with improved efficacy and a better safety profile. In this context, natural products can be a valuable source of substances with potential anticonvulsant activity. In the present study, we tested the anticonvulsant potential of Caryocar coriaceum Wittm., a plant native from the Brazilian Cerrado biome (tropical savanna ecoregion).
Adult male C57BL/6 mice were treated with increasing doses of the fixed oil obtained from the pulp of Caryocar coriaceum Wittm. Seizure activity was induced by PTZ (60 mg/kg, i.p.), and evaluated by behavioral and electrographic methods. Potential adverse effects were investigated in the open-field, rotarod, forced swim, or object recognition tests. The antioxidant potential of the oil was evaluated by the DPPH scavenging assay.
Administration of the oil at the dose of 100 mg/kg increased the latency for the first myoclonic jerk and the first generalized tonic-clonic seizures. The duration of generalized convulsions induced by PTZ was not altered. No significant behavioral adverse effects were detected in the open-field, rotarod, forced swim, or object recognition tests. Interestingly, a significant antioxidant activity of Caryocar coriaceum Wittm. fixed pulp oil was detected in the DPPH scavenging assay.
Natural products can be a valuable source of substances with potential anticonvulsant activity and improved safety profile. Further studies are needed to evaluate the mechanisms underlying the anticonvulsant effects of Caryocar coriaceum Wittm. fixed pulp oil as well as the potential of the oil as a source of new anticonvulsant compounds.
癫痫是一种常见的脑部疾病，也是全球主要的公共卫生问题。目前可用的药物治疗对大量癫痫患者发作的控制仍不充分。因此，需要发现疗效更好且安全性更高的新型抗惊厥方法。在此背景下，天然产物可能是具有潜在抗惊厥活性物质的宝贵来源。在本研究中，我们测试了来自巴西塞拉多生物群落（热带稀树草原生态区）的植物维特密花嘉赐树（Caryocar coriaceum Wittm.）的抗惊厥潜力。
用从维特密花嘉赐树果肉中提取的固定油，以递增剂量对成年雄性C57BL/6小鼠进行处理。通过腹腔注射戊四氮（60 mg/kg）诱导癫痫发作，并通过行为学和脑电图方法进行评估。在旷场试验、转棒试验、强迫游泳试验或物体识别试验中研究潜在的不良反应。通过DPPH清除试验评估该油的抗氧化潜力。
以100 mg/kg的剂量给药该油，可延长首次肌阵挛抽搐和首次全身性强直阵挛发作的潜伏期。戊四氮诱导的全身性惊厥持续时间未改变。在旷场试验、转棒试验、强迫游泳试验或物体识别试验中未检测到明显的行为不良反应。有趣的是，在DPPH清除试验中检测到维特密花嘉赐树固定果肉油具有显著的抗氧化活性。
天然产物可能是具有潜在抗惊厥活性且安全性更高的物质的宝贵来源。需要进一步研究来评估维特密花嘉赐树固定果肉油抗惊厥作用的潜在机制，以及该油作为新型抗惊厥化合物来源的潜力。
