Choi Kwang-Ho, Kwon O Sang, Cho Seong Jin, Lee Sanghun, Kang Seok-Yun, Ryu Yeon Hee
KM Fundamental Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-Gu, Daejeon, 305-811, South Korea.
BMC Complement Altern Med. 2017 May 3;17(1):246. doi: 10.1186/s12906-017-1754-8.
The P300 is a major index used to evaluate improvements in brain function. Although a few studies have reported evaluating the effectiveness of manual acupuncture or electro-acupuncture by monitoring the P300, research in this field is not yet very active. The aim of this study was to investigate the effects of periodic low-frequency electrical stimulation applied to BL62 and KI6 on brain activity by analyzing the P300.
The study was conducted as a randomized double-blind test of 55 subjects in their 50s, including 26 males and 29 females. Each subject received 12 sessions of stimulation over a one-month period. In each session, low-frequency electrical stimulation at an average of 24 μA and 2 Hz was applied to the acupuncture points BL62 and KI6, and event-related potentials (ERPs) were measured before the first session and after the last session of the electrical stimulation.
The results of a chi-square test indicated that the double-blind test was conducted correctly. Compared to the Sham group, all the subjects in the Real stimulation group showed a tendency toward a decreasing P300 latency and increasing P300 amplitude after all 12 sessions of stimulation. In the women, the amplitude significantly increased at Fz, Fcz, Cz, Cpz, and Pz.
With this experiment, the low-frequency electrical stimulation of two acupuncture points (BL62 and K16) was confirmed to have a positive influence on the prevention of natural cerebral aging.
This study was registered at the Clinical Research Information Service (CRIS) of the National Research Institute of Health ( https://cris.nih.go.kr/cris/search/search_result_st01_en.jsp? , Registration Number: KCT0001940). The date of registration was June 9, 2016.
P300是用于评估脑功能改善情况的一项主要指标。尽管有一些研究报告称通过监测P300来评估手针或电针的效果，但该领域的研究尚不十分活跃。本研究的目的是通过分析P300来探究对膀胱经62穴和肾经6穴进行周期性低频电刺激对脑活动的影响。
本研究对55名50多岁的受试者进行了随机双盲试验，其中男性26名，女性29名。每位受试者在一个月内接受12次刺激。每次刺激时，对膀胱经62穴和肾经6穴施加平均24μA、2Hz的低频电刺激，并在电刺激的第一次治疗前和最后一次治疗后测量事件相关电位（ERP）。
卡方检验结果表明双盲试验进行正确。与假刺激组相比，真实刺激组的所有受试者在全部12次刺激后均呈现出P300潜伏期缩短、P300波幅增大的趋势。在女性中，Fz、Fcz、Cz、Cpz和Pz处的波幅显著增大。
通过本实验证实，对两个穴位（膀胱经62穴和肾经6穴）进行低频电刺激对预防自然脑老化有积极影响。
本研究在韩国国立卫生研究院临床研究信息服务中心（CRIS）注册（https://cris.nih.go.kr/cris/search/search_result_st01_en.jsp? ，注册号：KCT0001940）。注册日期为2016年6月9日。
