An Xia, Zhang Jingyu, Liao Yiwen, Liu Lijun, Peng Dingxiang, Wang Bo
Key Laboratory of Crop Ecophysiology and Farming Systems in the Middle Reaches of the Yangtze River Ministry of Agriculture, College of Plant Science and Technology Huazhong Agricultural University Wuhan China.
Flower Research and Development Centre Zhejiang Academy of Agricultural Sciences Hangzhou China.
FEBS Open Bio. 2017 Mar 31;7(5):636-644. doi: 10.1002/2211-5463.12191. eCollection 2017 May.
Ramie is an economically important industrial fiber crop widely planted in China, India, and other Southeast Asian and Pacific Rim countries. It plays an important role in China's economy, where ramie farming, industry, and trade provide livelihood support to about five million people. However, poor fiber production resulting from leaf senescence and leaf abscission is a significant problem. In this study, we report the successful production of transgenic ramie plants which delayed leaf senescence and enhanced biomass. Transgenic ramie plants were obtained via transformation with the strain harboring the binary vector pSG529 containing the isopentyl transferase () gene under control of the SAG12 promoter (P- construct). strain EHA105 was used for the midrib explant transformation. The transformation frequency was 28.29%. Southern blot confirmed the integration of 1-4 copies of the gene into the ramie genome in the tested lines. At the fiber maturation stage, the transgenic plants had higher photosynthesis rates, chlorophyll content (SPAD values), and stronger resistance to exogenous ethylene compared with wild-type plants.
苎麻是一种具有重要经济价值的工业纤维作物，在中国、印度以及其他东南亚和环太平洋国家广泛种植。它在中国经济中发挥着重要作用，苎麻种植、产业和贸易为约500万人提供了生计支持。然而，叶片衰老和脱落导致纤维产量低下是一个重大问题。在本研究中，我们报道了成功培育出延迟叶片衰老并增加生物量的转基因苎麻植株。通过用携带二元载体pSG529的菌株进行转化获得转基因苎麻植株，该二元载体含有在SAG12启动子（P-构建体）控制下的异戊烯基转移酶（）基因。菌株EHA105用于中脉外植体转化。转化频率为28.29%。Southern杂交证实了在测试品系中1-4个拷贝的基因整合到苎麻基因组中。在纤维成熟阶段，与野生型植株相比，转基因植株具有更高的光合速率、叶绿素含量（SPAD值）以及更强的对外源乙烯的抗性。
