Meissner G, Rousseau E, Lai F A, Liu Q Y, Anderson K A
Department of Biochemistry, University of North Carolina, Chapel Hill 27599.
Mol Cell Biochem. 1988 Jul-Aug;82(1-2):59-65. doi: 10.1007/BF00242517.
Rapid mixing-vesicle ion flux and planar lipid bilayer-single channel measurements have shown that a high-conductance, ligand-gated Ca2+ release channel is present in 'heavy', junctional-derived membrane fractions of skeletal and cardiac muscle sarcoplasmic reticulum. Using the release channel-specific probe, ryanodine, a 30S protein complex composed of polypeptides of Mr approximately 400,000 has been isolated from cardiac and skeletal muscle. Reconstitution of the complex into planar lipid bilayers has revealed a Ca2+ conductance with properties characteristic of the native Ca2+ release channel.
