一种基于ATP的使用96孔板检测动物非洲锥虫活力的荧光素酶检测法。
An ATP-Based Luciferase Viability Assay for Animal African Trypanosomes Using a 96-Well Plate.
作者信息
Suganuma Keisuke, Molefe Nthatisi Innocentia, Inoue Noboru
机构信息
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, 080-8555, Japan.
出版信息
Methods Mol Biol. 2017;1601:89-95. doi: 10.1007/978-1-4939-6960-9_8.
PMID:28470520
Abstract
Cell viability assays using multi-well cell culture plates are frequently used for in vitro drug screening. We herein describe an ATP-based luciferase viability assay for animal African trypanosomes using a 96-well plate. This assay could be further applied to the screening of novel compounds for the treatment of animal African trypanosomiasis.
摘要
使用多孔细胞培养板进行的细胞活力测定常用于体外药物筛选。我们在此描述一种基于ATP的荧光素酶活力测定方法，用于使用96孔板检测动物非洲锥虫。该测定方法可进一步应用于筛选治疗动物非洲锥虫病的新型化合物。
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