Korneva Yulia S, Dorosevich Alexander E, Maryakhina Valeriya S
Department of Pathological Anatomy, Smolensk State Medical University, 28 Krupskoy st., Smolensk, 214019, Russia.
Smolensk Regional Institute of Pathology, 27 Gagarina av., Smolensk, 214020, Russia.
Lasers Surg Med. 2017 Oct;49(8):763-766. doi: 10.1002/lsm.22683. Epub 2017 May 4.
Changes in the biochemical composition of the tissue during colon cancer progression usually precede morphological changes registered by light microscopy. These changes are very sensitive and may be used for diagnostics in difficult cases, when it is impossible to obtain sufficient amount of material during colonoscopy. The aim of the study is analysis of spectral characteristics of sporadic adenomas and tumors in different parts of colon for improving tumors diagnostics in disputable cases.
The spectra of fluorescence excitation of histological sections from 78 patients with colon cancer (adenocarcinoma) and colonic adenomas of different localizations were measured.
The spectra of fluorescence excitation of all types of adenomas as well as adenocarcinoma have two maxima at 260/270 nm and at 330/340 nm. The first maximum is primarily defined by tryptophan and phenylalanin containing peptides, one of them is glucagon. The second maximum is mainly defined by collagen in stroma. Progression of precancer lesions to advanced cancer leads to increase of NADH concentration impacting on the second maximum of spectra. However, spectra of all types of the investigated lesions have peculiarities depending on localization. At odds to the previous data about similarities between distal colon and rectum, our results demonstrate similar spectra for proximal colon and rectum due to some similarities in morphological and, as a consequence, biochemical composition. Tumor can be detected by spectral techniques on histological slides even if the specimen contains very few tumorous cells in stroma.
Biochemical changes and their similarities for precancer lesions and advanced colon cancer have described. Peculiarities of spectral data for different parts of colon may change the previous opinion about similar mechanisms of cancerogenesis for distal colon and rectum. Moreover, investigation of tissue specimen obtained for histological examination and containing lack of malignant epithelial cells in massive stroma does not interfere with analysis due to specific disproportion of spectrum maxima. Lasers Surg. Med. 49:763-766, 2017. © 2017 Wiley Periodicals, Inc.
在结肠癌进展过程中，组织的生化成分变化通常先于光学显微镜下记录到的形态学变化。这些变化非常敏感，在结肠镜检查无法获取足够组织材料的疑难病例中可用于诊断。本研究的目的是分析结肠不同部位散发性腺瘤和肿瘤的光谱特征，以改善疑难病例中的肿瘤诊断。
测量了78例结肠癌（腺癌）及不同部位结肠腺瘤患者组织切片的荧光激发光谱。
所有类型的腺瘤以及腺癌的荧光激发光谱在260/270nm和330/340nm处有两个峰值。第一个峰值主要由含色氨酸和苯丙氨酸的肽类决定，其中之一是胰高血糖素。第二个峰值主要由基质中的胶原蛋白决定。癌前病变进展为进展期癌会导致烟酰胺腺嘌呤二核苷酸（NADH）浓度升高，影响光谱的第二个峰值。然而，所有类型的研究病变光谱都有取决于部位的特点。与之前关于远端结肠和直肠相似性的报道不同，我们的结果表明近端结肠和直肠的光谱相似，这是由于形态学以及由此导致的生化成分存在一些相似性。即使标本在基质中含有极少的肿瘤细胞，也可通过光谱技术在组织切片上检测到肿瘤。
描述了癌前病变和进展期结肠癌的生化变化及其相似性。结肠不同部位光谱数据的特点可能会改变之前关于远端结肠和直肠致癌机制相似的观点。此外，对用于组织学检查且在大量基质中缺乏恶性上皮细胞的组织标本进行研究，由于光谱峰值的特定差异，不会干扰分析。《激光外科与医学》49:763 - 766，2017年。©2017威利期刊公司
