Römer T J, Fitzmaurice M, Cothren R M, Richards-Kortum R, Petras R, Sivak M V, Kramer J R
Department of Cardiology, Cleveland Clinic Foundation, Ohio.
Am J Gastroenterol. 1995 Jan;90(1):81-7.
To determine what structures fluoresce and to what extent in normal colon and colonic adenomas to fully exploit laser-induced fluorescence spectroscopy as a tool for the diagnosis of dysplasia at endoscopy.
Unstained frozen sections of normal colon and colonic adenomas were studied by fluorescence microscopy under 351-364-nm argon ion laser excitation. Tissue fluorescence was observed and compared to morphology in serial sections stained with hematoxylin and eosin (H&E), Movat pentachrome, mucicarmine, and oil red O.
In normal colon, fluorescence correlated morphologically with connective tissue fibers (principally collagen) in all layers of the bowel wall and with cytoplasmic granules within eosinophils present between the crypts in the lamina propria of the mucosa. Fluorescence of absorptive cells in normal crypts was very faint, and Goblet cells did not fluoresce. However, marked fluorescence was observed in the cytoplasms of dysplastic epithelial cells in the crypts of colonic adenomas. Fewer fluorescent connective tissue fibers were present in the lamina propria of colonic adenomas resulting in decreased fluorescence intensity as compared to that of normal colon. Fluorescent eosinophil granules were present in larger numbers in adenomas as compared with normal colon.
Laser-induced fluorescence in normal colon and colonic adenomas correlates with morphology. Previous reported differences in laser-induced fluorescence emission spectra of normal colon and colonic adenomas obtained in vitro and in vivo may be due to differences in the cytoplasmic fluorescence between the dysplastic epithelium in colonic adenomas and normal colonic epithelium. Laser-induced fluorescence spectroscopy may be useful in studying other forms of epithelial dysplasia such as that which occurs in ulcerative colitis.
确定正常结肠和结肠腺瘤中哪些结构会发生荧光以及荧光的程度，以充分利用激光诱导荧光光谱技术作为内镜检查中诊断发育异常的工具。
在351 - 364纳米氩离子激光激发下，通过荧光显微镜对正常结肠和结肠腺瘤的未染色冰冻切片进行研究。观察组织荧光，并与苏木精和伊红（H&E）、莫瓦特五色染色法、黏液卡红和油红O染色的连续切片中的形态进行比较。
在正常结肠中，荧光在形态上与肠壁各层的结缔组织纤维（主要是胶原蛋白）以及黏膜固有层隐窝之间存在的嗜酸性粒细胞内的细胞质颗粒相关。正常隐窝中吸收细胞的荧光非常微弱，杯状细胞不发荧光。然而，在结肠腺瘤隐窝中发育异常的上皮细胞的细胞质中观察到明显的荧光。与正常结肠相比，结肠腺瘤固有层中荧光结缔组织纤维较少，导致荧光强度降低。与正常结肠相比，腺瘤中荧光嗜酸性粒细胞颗粒数量更多。
正常结肠和结肠腺瘤中的激光诱导荧光与形态相关。先前报道的在体外和体内获得的正常结肠和结肠腺瘤的激光诱导荧光发射光谱的差异，可能是由于结肠腺瘤中发育异常的上皮细胞与正常结肠上皮细胞之间的细胞质荧光差异所致。激光诱导荧光光谱技术可能有助于研究其他形式的上皮发育异常，如溃疡性结肠炎中发生的发育异常。
