Delamere N A, Paterson C A, Hensley S
Department of Ophthalmology, University of Louisville, Ky.
Ophthalmic Res. 1988;20(3):200-4. doi: 10.1159/000266586.
Rabbit lenses exposed to 0.5 mM hydrogen peroxide for 1 h and then incubated for an additional 19 h demonstrated small yet significant changes in electrolyte balance. Active cation pumping by the lens, as determined by 86Rb uptake studies, was partially impaired following 1 h hydrogen peroxide exposure; the cation pump further deteriorated during the subsequent 19-hour incubation. Passive membrane permeability of the lens, measured by 86Rb efflux, was elevated following 1 h hydrogen peroxide but no further increase in leakiness was observed during the subsequent 19-hour incubation. These findings suggest that even relatively brief exposure to oxidative damage initiates a chain of events from which the lens is unable to recover.
将兔晶状体暴露于0.5 mM过氧化氢中1小时，然后再孵育19小时，结果显示电解质平衡发生了微小但显著的变化。通过86Rb摄取研究确定，晶状体的活性阳离子泵在暴露于过氧化氢1小时后部分受损；在随后的19小时孵育过程中，阳离子泵进一步恶化。通过86Rb外流测量的晶状体被动膜通透性在过氧化氢暴露1小时后升高，但在随后的19小时孵育过程中未观察到渗漏进一步增加。这些发现表明，即使是相对短暂的氧化损伤暴露也会引发一系列晶状体无法恢复的事件。
