Voss J H, Roizman B
Marjorie B. Kovler Viral Oncology Laboratories, University of Chicago, IL 60637.
Proc Natl Acad Sci U S A. 1988 Nov;85(22):8454-8. doi: 10.1073/pnas.85.22.8454.
In cells infected with herpes simplex virus 1 the domains of the two identical origins of viral DNA synthesis in the S component (Oris) are transcribed in two 5'-coterminal RNAs. OriSRNA1, synthesized early in infection and also in the absence of protein synthesis by infected cells, runs antisense to and terminates at the transcription initiation site for alpha genes 22 or 47. OriSRNA2, reported earlier, was detected in cells 14 hr after infection; it extends across the origin and is coterminal with the alpha 4 mRNA. Whereas the OriSRNA2 of herpes simplex virus 1 (HSV-1) contains an open reading frame, the corresponding herpes simplex virus 2 (HSV-2) transcript does not. However, in the two viruses, the transcription initiation sites are 260 (HSV-1) to 180 (HSV-2) nucleotides distant from the corresponding origins of DNA synthesis.
在感染单纯疱疹病毒1的细胞中，S组分（Oris）中两个相同的病毒DNA合成起始位点的结构域被转录为两个5'-共末端RNA。OriSRNA1在感染早期合成，也可在感染细胞无蛋白质合成的情况下合成，其转录方向与α基因22或47的转录起始位点相反，并在该位点终止。先前报道的OriSRNA2在感染后14小时的细胞中被检测到；它延伸穿过起始位点，并与α4 mRNA共末端。单纯疱疹病毒1（HSV-1）的OriSRNA2含有一个开放阅读框，而相应的单纯疱疹病毒2（HSV-2）转录本则没有。然而，在这两种病毒中，转录起始位点与相应的DNA合成起始位点相距260个核苷酸（HSV-1）至180个核苷酸（HSV-2）。
