Dean H J, Cheung A K
Virology Swine Research Unit, U.S. Department of Agriculture, Ames, Iowa 50010.
J Virol. 1993 Oct;67(10):5955-61. doi: 10.1128/JVI.67.10.5955-5961.1993.
We have determined the nucleotide sequence and transcription pattern of a group of open reading frames of pseudorabies virus (PRV), which are located at the right end of the BamHI-G fragment from 0.664 to 0.695 map units in the unique long region of the genome. Nucleotide sequence analysis revealed four open reading frames. The first three correspond in genome location to the herpes simplex virus type 1 (HSV-1) open reading frames UL1, which codes for glycoprotein L (gL); UL2, which codes for a uracil-DNA glycosylase; and UL3, which codes for a polypeptide of unknown function. The fourth open reading frame, UL3.5, is not present in the HSV-1 genome. Northern (RNA) blot analysis with oligonucleotide and cDNA probes revealed four abundant mRNA species of 3.3, 2.7, 1.8, and 0.9 kb, which are likely to yield polypeptides encoded by the UL1, -2, -3, and -3.5 open reading frames, respectively. All four transcripts were of the early-late kinetic class, transcribed in the same direction, and 3' coterminal. The UL2 and UL3 genes of PRV and HSV-1 have significant amino acid sequence homology, while the UL1 genes are positional homologs and the UL3.5 gene is unique to PRV.
我们已经确定了一组伪狂犬病病毒（PRV）开放阅读框的核苷酸序列和转录模式，这些开放阅读框位于基因组独特长区域中BamHI - G片段右端，从0.664到0.695个图谱单位处。核苷酸序列分析揭示了四个开放阅读框。前三个在基因组位置上分别对应于1型单纯疱疹病毒（HSV - 1）的开放阅读框UL1（编码糖蛋白L，即gL）、UL2（编码尿嘧啶 - DNA糖基化酶）和UL3（编码功能未知的多肽）。第四个开放阅读框UL3.5在HSV - 1基因组中不存在。用寡核苷酸和cDNA探针进行的Northern（RNA）印迹分析揭示了四种丰富的mRNA种类，大小分别为3.3、2.7、1.8和0.9 kb，它们可能分别产生由UL1、 - 2、 - 3和 - 3.5开放阅读框编码的多肽。所有这四种转录本都属于早期 - 晚期动力学类型，转录方向相同，并且3'端共末端。PRV和HSV - 1的UL2和UL3基因具有显著的氨基酸序列同源性，而UL1基因是位置同源物，UL3.5基因是PRV特有的。
