Xie Jun, Zhu Suhui, Dai Qing, Lu Jianrong, Chen Jianzhou, Li Guannan, Wu Han, Li Ran, Huang Wei, Xu Biao, Xu Wei
Department of Cardiology, Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.
Medicine (Baltimore). 2017 May;96(18):e6806. doi: 10.1097/MD.0000000000006806.
The mechanism underlying thrombosis in atrial fibrillation (AF) is not yet clearly understood. Oncostatin M (OSM), as a member of IL-6 family, is involved in atherosclerosis-mediated thrombosis. The present study hypothesizes that OSM and its downstream factors play a role in thrombogenesis in AF.The specimens of left atrial appendages collected from patients with rheumatic mitral stenosis who underwent valve replacement were divided into 3 groups: sinus rhythm, AF(+)/thrombus(-), and AF(+)/thrombus(+) group. The macrophage infiltration in atrial tissue was assessed by immunohistochemistry, and the amount of OSM, tissue factor (TF), and tissue factor pathway inhibitors (TFPIs) was detected by Western blot.The infiltration of the M1 macrophages was significantly increased in the AF with thrombus group compared with the sinus rhythm group (P = .03). Moreover, the expression of OSM and TF was much higher in the AF with thrombus group compared with the sinus rhythm group (P = .02, .009, respectively) while the TFPI was decreased in the AF with thrombus group (P = .04).OSM might be correlated with thrombosis in patients with AF mediated by TF and TFPI.
心房颤动（AF）中血栓形成的潜在机制尚未完全明确。抑瘤素M（OSM）作为白细胞介素-6家族的一员，参与动脉粥样硬化介导的血栓形成。本研究假设OSM及其下游因子在AF的血栓形成中起作用。从接受瓣膜置换术的风湿性二尖瓣狭窄患者收集的左心耳标本分为3组：窦性心律组、AF（+）/血栓（-）组和AF（+）/血栓（+）组。通过免疫组织化学评估心房组织中的巨噬细胞浸润情况，并用蛋白质印迹法检测OSM、组织因子（TF）和组织因子途径抑制剂（TFPIs）的含量。与窦性心律组相比，血栓形成的AF组中M1巨噬细胞的浸润显著增加（P = 0.03）。此外，与窦性心律组相比，血栓形成的AF组中OSM和TF的表达更高（分别为P = 0.02、0.009），而血栓形成的AF组中TFPI降低（P = 0.04）。OSM可能与TF和TFPI介导的AF患者血栓形成相关。
