Yoshikawa M, Nakasa T, Ishikawa M, Adachi N, Ochi M
Department of Orthopaedic Surgery, Institute of Biomedical & Health Sciences, Hiroshima University, Japan.
Department of Orthopaedic Surgery, Institute of Biomedical & Health Sciences, Hiroshima University, Japan
Bone Joint Res. 2017 May;6(5):277-283. doi: 10.1302/2046-3758.65.BJR-2016-0187.R1.
Regenerative medicine is an emerging field aimed at the repair and regeneration of various tissues. To this end, cytokines (CKs), growth factors (GFs), and stem/progenitor cells have been applied in this field. However, obtaining and preparing these candidates requires invasive, costly, and time-consuming procedures. We hypothesised that skeletal muscle could be a favorable candidate tissue for the concept of a point-of-care approach. The purpose of this study was to characterize and confirm the biological potential of skeletal muscle supernatant for use in regenerative medicine.
Semitendinosus muscle was used after harvesting tendon from patients who underwent anterior cruciate ligament reconstructions. A total of 500 milligrams of stripped muscle was minced and mixed with 1 mL of saline. The collected supernatant was analysed by enzyme-linked immunosorbent assay (ELISA) and flow cytometry. The biological effects of the supernatant on cell proliferation, osteogenesis, and angiogenesis in vitro were evaluated using human mesenchymal stem cells (hMSCs) and human umbilical cord vein endothelial cells (HUVECs).
The supernatant contained several GFs/CKs, with especially high levels of basic fibroblast growth factor, and CD34+ cells as the stem/progenitor cell fraction. With regard to biological potential, we confirmed that cell proliferation, osteoinduction, and angiogenesis in hMSCs and HUVECs were enhanced by the supernatant.
The current study demonstrates the potential of a new point-of-care strategy for regenerative medicine using skeletal muscle supernatant. This attractive approach and readily-available material could be a promising option for tissue repair/regeneration in the clinical setting.: M. Yoshikawa, T. Nakasa, M. Ishikawa, N. Adachi, M. Ochi. Evaluation of autologous skeletal muscle-derived factors for regenerative medicine applications. 2017;6:277-283. DOI: 10.1302/2046-3758.65.BJR-2016-0187.R1.
再生医学是一个旨在修复和再生各种组织的新兴领域。为此，细胞因子（CKs）、生长因子（GFs）和干/祖细胞已被应用于该领域。然而，获取和制备这些候选物需要侵入性、昂贵且耗时的程序。我们假设骨骼肌可能是即时医疗方法概念的理想候选组织。本研究的目的是表征和确认骨骼肌上清液在再生医学中的生物学潜力。
从接受前交叉韧带重建的患者身上获取肌腱后使用半腱肌。将总共500毫克的去肌腱肌肉切碎并与1毫升生理盐水混合。通过酶联免疫吸附测定（ELISA）和流式细胞术分析收集的上清液。使用人间充质干细胞（hMSCs）和人脐静脉内皮细胞（HUVECs）评估上清液对体外细胞增殖、成骨和血管生成的生物学作用。
上清液含有多种GFs/CKs，其中碱性成纤维细胞生长因子水平特别高，并且CD34 +细胞作为干/祖细胞部分。关于生物学潜力，我们证实上清液可增强hMSCs和HUVECs中的细胞增殖、骨诱导和血管生成。
当前研究证明了使用骨骼肌上清液进行再生医学的新即时医疗策略的潜力。这种有吸引力的方法和易于获得的材料可能是临床环境中组织修复/再生的有前途的选择。：M.Yoshikawa，T.Nakasa，M.Ishikawa，N.Adachi，M.Ochi。自体骨骼肌衍生因子在再生医学应用中的评估。2017；6：277 - 283。DOI：10.1302/2046 - 3758.65.BJR - 2016 - 0187.R1。
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