School of Chemical and Biological Engineering Seoul National University Seoul 151-744, Korea.
Exp Mol Med. 2011 Nov 30;43(11):622-9. doi: 10.3858/emm.2011.43.11.070.
Platelet-rich plasma (PRP) contains growth factors that promote tissue regeneration. Previously, we showed that heparin-conjugated fibrin (HCF) exerts the sustained release of growth factors with affinity for heparin. Here, we hypothesize that treatment of skin wound with a mixture of PRP and HCF exerts sustained release of several growth factors contained in PRP and promotes skin wound healing. The release of fibroblast growth factor 2, platelet-derived growth factor-BB, and vascular endothelial growth factor contained in PRP from HCF was sustained for a longer period than those from PRP, calcium-activated PRP (C-PRP), or a mixture of fibrin and PRP (F-PRP). Treatment of full-thickness skin wounds in mice with HCF-PRP resulted in much faster wound closure as well as dermal and epidermal regeneration at day 12 compared to treatment with either C-PRP or F-PRP. Enhanced skin regeneration observed in HCF-PRP group may have been at least partially due to enhanced angiogenesis in the wound beds. Therefore, this method could be useful for skin wound treatment.
富含血小板的血浆 (PRP) 含有促进组织再生的生长因子。我们之前曾表明，肝素结合纤维蛋白 (HCF) 具有肝素亲和力，可实现生长因子的持续释放。在这里，我们假设用 PRP 和 HCF 的混合物治疗皮肤伤口会持续释放 PRP 中包含的几种生长因子，并促进皮肤伤口愈合。HCF 从 PRP 中持续释放的成纤维细胞生长因子 2、血小板衍生生长因子-BB 和血管内皮生长因子的时间比 PRP、钙激活 PRP (C-PRP) 或纤维蛋白和 PRP 的混合物 (F-PRP) 更长。与 C-PRP 或 F-PRP 治疗相比，用 HCF-PRP 治疗小鼠全层皮肤伤口可使伤口更快闭合，并在第 12 天促进真皮和表皮再生。HCF-PRP 组观察到的增强的皮肤再生至少部分可能是由于伤口床中的血管生成增强所致。因此，该方法可能对皮肤伤口治疗有用。
