一种用于检测马血清中抗马鼻肺炎病毒（EHV-1）抗体的放射免疫扩散酶测定法。

A radial immunodiffusion enzyme assay for detection of antibody to equine rhinopneumonitis virus (EHV-1) in horse serum.

作者信息

Gradil C, Joo H S

机构信息

Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.

出版信息

Vet Microbiol. 1988 Aug;17(4):315-22. doi: 10.1016/0378-1135(88)90045-4.

DOI:10.1016/0378-1135(88)90045-4

PMID:2847396

Abstract

A radial immunodiffusion enzyme assay (RIDEA) was developed for detection and quantitation of antibodies to equine herpes virus-1 (EHV-1) in horse sera. The detection and quantitation of EHV-1 antibody levels were based on the diameter of the radial diffusion zone of specific antibody in each serum sample reacting with EHV-1 antigen. The circular zone was made visible using peroxidase-conjugated rabbit anti-horse immunoglobulin G and a substrate containing hydrogen peroxide. The results of the RIDEA were compared with those of virus neutralization (VN) and enzyme-linked immunosorbent assay (ELISA) and found to be highly correlated. The relative sensitivity and specificity (percentage of agreement with VN test) were found to be 98.2 and 92.5%, respectively. Because the test procedure is relatively easy to perform, the RIDEA could be used as a field test to detect antibodies to EHV-1 in horses.

摘要

已开发出一种放射免疫扩散酶测定法（RIDEA），用于检测和定量马血清中抗马疱疹病毒1型（EHV-1）的抗体。EHV-1抗体水平的检测和定量基于每个血清样本中特异性抗体与EHV-1抗原反应的放射扩散区直径。使用过氧化物酶偶联的兔抗马免疫球蛋白G和含过氧化氢的底物使圆形区域可见。将RIDEA的结果与病毒中和试验（VN）和酶联免疫吸附测定（ELISA）的结果进行比较，发现高度相关。相对敏感性和特异性（与VN试验一致的百分比）分别为98.2%和92.5%。由于该检测程序相对易于执行，RIDEA可作为一种现场检测方法，用于检测马体内抗EHV-1的抗体。