通过酶联免疫吸附测定法检测马疱疹病毒1型抗原和特异性抗体。

Detection of equine herpesvirus-1 antigen and the specific antibody by enzyme-linked immunosorbent assay.

作者信息

Dutta S K, Talbot N C, Myrup A C

出版信息

Am J Vet Res. 1983 Oct;44(10):1930-4.

Abstract

Enzyme-linked immunosorbent assays (ELISA) were developed to detect equine herpesvirus-1 (EHV-1) antigens and specific antibodies. Detection of EHV-1 antigens was done by a 4-layer ELISA. In inoculated cell cultures, EHV-1 cell antigen was detected after postinoculation (PI) hour 4, reached approximately twice the value of EHV-1 viral antigen (extra-cellular virus) in PI hour 18, and peaked in PI hour 24, whereas EHV-1 viral antigen appeared after PI hour 12, increased steadily, and peaked higher than EHV-1 cell antigen in 24 hours. The ELISA titer and infectivity titer of 24-hour PI cultures were 200 and 9.2 X 10(5) plaque-forming units/0.1 ml, respectively. Equine anti-EHV-1 antibody (EAEHV-1) from experimentally inoculated pony foals and rabbit anti-EHV-1 antibody (RAEHV-1) were detected by indirect ELISA and direct ELISA, respectively. The RAEHV-1 had ELISA titers of 665,000 and 102,000 when rabbits were immunized with EHV-1 infected cell culture lysate and with sucrose-purified virus, respectively. The corresponding plaque-reduction virus-neutralization titers were 270 and 150 in the absence of complement and were 6,200 and 3,200 in the presence of complement. The EAEHV-1 had a mean ELISA titer of 60,000, and the corresponding mean virus-neutralization titer in the absence of complement was 34 and that in the presence of complement was 2,142.

摘要

酶联免疫吸附测定（ELISA）被用于检测马疱疹病毒1型（EHV - 1）抗原和特异性抗体。EHV - 1抗原的检测通过四层ELISA进行。在接种的细胞培养物中，接种后（PI）4小时可检测到EHV - 1细胞抗原，在PI 18小时达到约为EHV - 1病毒抗原（细胞外病毒）值的两倍，并在PI 24小时达到峰值，而EHV - 1病毒抗原在PI 12小时后出现，稳步增加，并在24小时达到高于EHV - 1细胞抗原的峰值。PI 24小时培养物的ELISA滴度和感染性滴度分别为200和9.2×10⁵蚀斑形成单位/0.1 ml。分别通过间接ELISA和直接ELISA检测来自实验接种小马驹的马抗EHV - 1抗体（EAEHV - 1）和兔抗EHV - 1抗体（RAEHV - 1）。当用EHV - 1感染的细胞培养物裂解物和蔗糖纯化病毒免疫兔子时，RAEHV - 1的ELISA滴度分别为665,000和102,000。在无补体时相应的蚀斑减少病毒中和滴度为270和150，在有补体时为6,200和3,200。EAEHV - 1的平均ELISA滴度为60,000，在无补体时相应的平均病毒中和滴度为34，在有补体时为2,142。