Liu Cai-Hong, DU Li
Department of Prosthodontics, Yan'an University Affiliated Hospital. Yan'an 716000, Shaanxi Province, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2017 Feb;26(1):42-47.
To explore the effects and molecular mechanisms of ginsenoside Rg1 on the proliferation and migration of human periodontal ligament cells (HPDLCs) under nicotine stress.
HPDLCs were isolated and cultured by method of explant cell culture. The cells were cultured under nicotine stress for 7 days, and treated respectively with ginsenoside Rg1 (0.01 μmol/L), ginsenoside Rg1 and LY294002 (PI3K inhibitor, 0.5 μmol/L), ginsenoside Rg1 and Tricirbine (Akt inhibitor, 5 μmol/L), ginsenoside Rg1 and L-NAME (Akt inhibitor, 1 mmol/L) from 3rd day after nicotine stress to 7th day. MTT assay and Transwell assay were used to evaluate the proliferation and migration of HPDLCs in each group. Western blot and quantitative real-time PCR methods were used for testing the changes of PI3K/Akt/eNOS signaling expression. SPSS 20.0 software package was used for statistical analysis.
The proliferation and migration were significantly inhibited by nicotine treatment. PI3K levels were upregulated, but Akt1/2 and eNOS levels were remarkedly reduced by nicotine. Ginsenoside Rg1 attenuated the effects of nicotine on proliferation, migration and Akt/eNOS signaling. Tricirbine and L-NAME could reduce the inhibitory effects of ginsenoside Rg1 toward nicotine.
Ginsenoside Rg1 regulates the proliferation and migration of HPDLCs under nicotine stress via Akt/eNOS signaling.
探讨人参皂苷Rg1在尼古丁应激下对人牙周膜细胞（HPDLCs）增殖和迁移的影响及其分子机制。
采用组织块细胞培养法分离培养HPDLCs。细胞在尼古丁应激下培养7天，从尼古丁应激后第3天至第7天分别用人参皂苷Rg1（0.01μmol/L）、人参皂苷Rg1与LY294002（PI3K抑制剂，0.5μmol/L）、人参皂苷Rg1与曲西立滨（Akt抑制剂，5μmol/L）、人参皂苷Rg1与L-NAME（Akt抑制剂，1mmol/L）处理。采用MTT法和Transwell法评估各组HPDLCs的增殖和迁移情况。采用蛋白质免疫印迹法和实时定量PCR法检测PI3K/Akt/eNOS信号表达的变化。使用SPSS 20.0软件包进行统计分析。
尼古丁处理显著抑制细胞增殖和迁移。尼古丁使PI3K水平上调，但Akt1/2和eNOS水平显著降低。人参皂苷Rg1减弱了尼古丁对增殖、迁移和Akt/eNOS信号的影响。曲西立滨和L-NAME可降低人参皂苷Rg1对尼古丁的抑制作用。
人参皂苷Rg1在尼古丁应激下通过Akt/eNOS信号调节HPDLCs的增殖和迁移。
