Stomatology Center, Gansu Provincial Hospital, Lanzhou City 730000, China.
Department of Urology, Gansu Provincial Hospital, Lanzhou City 730000, China.
Biomed Res Int. 2022 Jun 29;2022:7576359. doi: 10.1155/2022/7576359. eCollection 2022.
, . To investigate the effect of ginsenoside Rg1 on the biological activity of primary cultured human periodontal ligament cells (PDLC). . The effects of ginsenoside Rg1 on the proliferation activity, protein synthesis, and alkaline phosphatase (ALP) activity of primary cultured human periodontal ligament cells were investigated by thiazole blue (MTT) colorimetric method, Coomassie brilliant blue method, and enzyme kinetics method. The effect of ginsenoside Rg1 on cell cycle was detected by flow cytometry, and the cells were labeled with calcium ion-sensitive fluorescent probe Fluo3/AM, and the effect of ginsenoside Rg1 on intracellular free calcium concentration was detected by laser scanning confocal microscope. . Compared with the control group, the experimental groups of ginsenoside Rg1 at various concentrations could significantly promote cell proliferation, and the effect time was the longest in the concentration range of 0.01-0.05 mol/L;, Rg1 0.01umol/L and 0.05umol/L. The protein content in the 72-hour cell culture medium of the mol/L group was significantly higher than that of the control group; the ALP activity in the 72-hour cell culture medium of the Rg1 0.01 mol/L, 0.05 mol/L, and 0.1 mol/L groups was significantly higher than that of the control group; FCM assay showed that after 0.1 mol/L Rg1 for 48 hours, compared with the control group, the proportion of cells in the early stage of DNA synthesis (G1%) of PDLC was significantly reduced, while the proportion of cells in the DNA synthesis stage (S%) and the value of cell proliferation index PrI (S + G2M)% were significantly increased; Rg1 increased intracellular calcium in PDLC cells at first and then decreased and finally maintained at a slightly higher resting calcium level than before drug addition. . Ginsenoside Rg1 can increase the proliferation activity, protein synthesis, and alkaline phosphatase activity of periodontal ligament cells within a certain concentration range; Rg1 reduces the cells in G1 phase and increases cells in S phase of periodontal ligament fibroblasts. Change the concentration of free calcium ions in cells and promote more cells to enter a proliferative state.
研究人参皂甙 Rg1 对原代培养人牙周膜细胞（PDLC）生物学活性的影响。
采用噻唑蓝（MTT）比色法、考马斯亮蓝法和酶动力学法观察人参皂甙 Rg1 对原代培养人牙周膜细胞增殖活性、蛋白质合成和碱性磷酸酶（ALP）活性的影响，流式细胞术检测人参皂甙 Rg1 对细胞周期的影响，采用钙离子敏感荧光探针 Fluo3/AM 标记细胞，激光共聚焦显微镜检测人参皂甙 Rg1 对细胞内游离钙浓度的影响。
与对照组比较，各浓度人参皂甙 Rg1 实验组均能显著促进细胞增殖，且在 0.01~0.05 mol/L 浓度范围内作用时间最长；0.01、0.05 μmol/L Rg1 组细胞培养上清液中蛋白质含量明显高于对照组；0.01、0.05、0.1 mol/L Rg1 组细胞培养上清液中 ALP 活性明显高于对照组；FCM 检测显示，0.1 mol/L Rg1 作用 48 h 后，与对照组比较，PDLC 细胞 DNA 合成早期（G1%）细胞比例明显降低，而 DNA 合成期（S%）和细胞增殖指数 PrI（S+G2M）%细胞比例明显升高；Rg1 作用于 PDLC 细胞后，细胞内游离钙先升高后降低，最后维持在比加药前稍高的静息钙水平。
人参皂甙 Rg1 可在一定浓度范围内提高牙周膜细胞的增殖活性、蛋白质合成及 ALP 活性；Rg1 降低牙周膜成纤维细胞 G1 期细胞比例，增加 S 期细胞比例，改变细胞内游离钙浓度，促进更多细胞进入增殖状态。
