体外组装的牛轮状病毒核衣壳蛋白（VP6）管状物的结构。

The structure of tubes of bovine rotavirus nucleocapsid protein (VP6) assembled in vitro.

作者信息

Ready K F, Buko K M, Whippey P W, Alford W P, Bancroft J B

机构信息

Veterinary Infectious Disease Organization, Saskatoon, Saskatchewan, Canada.

出版信息

Virology. 1988 Nov;167(1):50-5. doi: 10.1016/0042-6822(88)90053-0.

DOI:10.1016/0042-6822(88)90053-0

PMID:2847425

Abstract

The structure of tubes of reassembled nucleocapsid protein (VP6) from bovine rotavirus (BRV) was determined using optical diffraction of electron micrographs. The tubes consist of a five-start helix of hexagons, with 38 hexagons per helix in a true repeat of three turns. The morphological subunits comprising the hexagons are probably elongated trimers. The structure of naturally occurring tubes (D. Chasey and J. Labram, 1983, J. Gen. Virol. 64, 863-872) was also examined and shown to be similar but not identical to that of tubes assembled in vitro. Considerations of the assembly process are discussed.

摘要

利用电子显微镜照片的光学衍射确定了来自牛轮状病毒（BRV）的重组核衣壳蛋白（VP6）管的结构。这些管由六边形的五重螺旋组成，每三圈的一个真正重复中，每个螺旋有38个六边形。构成六边形的形态学亚基可能是伸长的三聚体。还检查了天然存在的管的结构（D. Chasey和J. Labram，1983年，《普通病毒学杂志》64卷，863 - 872页），结果表明其与体外组装的管的结构相似但不完全相同。文中讨论了组装过程的相关因素。