Department of Endocrinology, the Fourth Hospital of Hebei Medical University, No. 12 Jiankang Road, Shijiazhuang, 050011, China.
Department of Vascular Surgery, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing, 100091, China.
Mol Cell Biochem. 2017 Sep;433(1-2):161-167. doi: 10.1007/s11010-017-3024-y. Epub 2017 May 4.
The therapeutic effect of transplantation of human umbilical cord blood cell-derived mononuclear cells (HUCMNCs) on treating in-stent restenosis (ISR) after endovascular interventional therapy (EIT) was evaluated in preclinical rabbit model of type 2 diabetes mellitus (T2DM)-related peripheral artery disease (PAD). HUCMNCs were transplanted to T2DM rabbits subjected to femoral artery occlusion surgery and received EIT. Serum concentration of soluble vascular endothelial cadherin (VE-cad) and plasma concentration of lipoprotein-associated phospholipase A2 (Lp-PLA2) were determined with enzyme-linked immunosorbent assay before and after the transplantation. The injury and the recovery of right femoral artery at stenting site were evaluated with Hematoxylin and Eosin (HE) staining. HUCMNCs purified from umbilical cord blood were 100% CD45 and 96.5% CD34 with round or oval morphology and adherent growth pattern. The soluble VE-cad and Lp-PLA2 were significantly attenuated after HUCMNC transplantation. The intimal area and the ratio between intimal area and medium film area in the dilated occlusion site were also dramatically decreased 4 weeks after receiving HUCMNCs. HUCMNC transplantation is effective in protecting vascular endothelial function and preventing ISR after EIT in T2DM rabbits suffering from PAD.
人脐血单个核细胞（HUCMNCs）移植治疗 2 型糖尿病（T2DM）相关外周动脉疾病（PAD）患者血管内介入治疗（EIT）后支架内再狭窄（ISR）的疗效在 2 型糖尿病兔 PAD 模型中进行了临床前评估。将 HUCMNCs 移植到接受股动脉闭塞手术和 EIT 的 T2DM 兔中。在移植前后，通过酶联免疫吸附试验测定血清可溶性血管内皮钙黏蛋白（VE-cad）浓度和血浆脂蛋白相关磷脂酶 A2（Lp-PLA2）浓度。用苏木精和伊红（HE）染色评估右股动脉支架部位的损伤和恢复情况。从脐血中纯化的 HUCMNCs 为 100% CD45 和 96.5% CD34，呈圆形或椭圆形形态，具有贴壁生长模式。HUCMNC 移植后，可溶性 VE-cad 和 Lp-PLA2 明显减弱。接受 HUCMNC 治疗 4 周后，扩张闭塞部位的内膜面积和内膜面积与中膜面积之比也显著减少。HUCMNC 移植可有效保护血管内皮功能，预防 T2DM 合并 PAD 患者 EIT 后 ISR。
