Weiler R, Fischer-Colbrie R, Schmid K W, Feichtinger H, Bussolati G, Grimelius L, Krisch K, Kerl H, O'Connor D, Winkler H
Department of Pharmacology, University of Innsbruck, Austria.
Am J Surg Pathol. 1988 Nov;12(11):877-84. doi: 10.1097/00000478-198811000-00008.
We investigated a variety of endocrine tumors for the presence of chromogranins A and B and secretogranin II. These antigens were identified by one- and two-dimensional immunoblotting and in some cases by immunohistochemistry. An antigen corresponding in electrophoretic behavior to adrenal chromogranin A was present in all types of tumors, including insulinomas, oat cell carcinomas, and Merkel cell tumors of the skin. Chromogranin B had a much more limited distribution. This antigen could not be detected in parathyroid adenomas, oat cell carcinomas, or Merkel cell tumors, either by immunoblotting and immunohistochemistry. The occurrence of secretogranin II was similar to that of chromogranin B, with the exception of a positive reaction in Merkel cell tumors. In benign pheochromocytomas, all three antigens were found consistently; whereas in two of three malignant pheochromocytomas, chromogranin B was absent. Our study establishes that in most cases chromogranins and secretogranin in tumors are identical to the adrenal antigens, but that these antigens are not always stored together. Chromogranin A is the most widely distributed marker for endocrine tumors.
我们研究了多种内分泌肿瘤中嗜铬粒蛋白A和B以及分泌粒蛋白II的存在情况。这些抗原通过一维及二维免疫印迹法进行鉴定，部分情况下还通过免疫组织化学法进行鉴定。在所有类型的肿瘤中均存在一种在电泳行为上与肾上腺嗜铬粒蛋白A相对应的抗原，包括胰岛素瘤、燕麦细胞癌以及皮肤默克尔细胞癌。嗜铬粒蛋白B的分布则更为有限。通过免疫印迹法和免疫组织化学法，在甲状旁腺腺瘤、燕麦细胞癌或默克尔细胞肿瘤中均未检测到该抗原。分泌粒蛋白II的出现情况与嗜铬粒蛋白B相似，但默克尔细胞肿瘤呈阳性反应。在良性嗜铬细胞瘤中，始终能发现所有这三种抗原；而在三分之二的恶性嗜铬细胞瘤中，不存在嗜铬粒蛋白B。我们的研究表明，在大多数情况下，肿瘤中的嗜铬粒蛋白和分泌粒蛋白与肾上腺抗原相同，但这些抗原并非总是一起储存。嗜铬粒蛋白A是内分泌肿瘤分布最广泛的标志物。
