de Fatima Ferreira Martins Edenilce, Magnone Larisa, Bessonart Martin, Costa Deliane Cristina, Dos Santos José Cláudio Epaminondas, Bazzoli Nilo, Nakayama Cintia Labussière, Luz Ronald Kennedy
Laboratório de Aquacultura da Escola de Veterinária da Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, MG, Brazil.
Laboratorio de Recursos Naturales, Instituto de Ecología y Ciencias Ambientales, Facultad de Ciencias, Universidad de la República, Iguá, 4225, Montevideo, Uruguay.
Anim Reprod Sci. 2017 Jun;181:167-174. doi: 10.1016/j.anireprosci.2017.04.005. Epub 2017 Apr 19.
The objective of this work was to evaluate the fatty acid and lipid composition of oocytes, newly hatched larvae (NHL), first feeding larvae (FFL) and muscle tissue of female Brycon orthotaenia broodstock. Total and polar lipid was significantly (P<0.05) abundant in oocytes and larvae in different stages of development. The lowest content (P<0.05) of total lipids was found in the muscles, whereas total lipid content of oocytes, NHL and FFL did not show any significant difference. Polar lipid content was different (P<0.05) between NHL and FFL. For the neutral the lowest values of C18:2n 6 occurred during the initial feeding period, whilst C20:4n 6 (AA) exhibited the highest percentage in FFL (P<0.05). C22:6n 3 (DHA) was highest (P<0.05) in FFL. The neutral lipid n-9 and n-6 was highest in muscle of females. The n-3HUFA was highest in NHL and in FFL, n-6HUFA was highest in FFL (P<0.05). The ratios of DHA/EPA were higher (P<0.05) in oocytes and FFL. In fatty acids of polar lipids, C20:5n 3 (EPA) did not show differences (P>0.05) between stages. C18:3n 3 was highest (P<0.05) in NHL and FFL. C20:4n 6 (AA) and C22:6n 3 (DHA) showed the highest percentages during the larval stages. The fatty acids n-3 series was significantly higher (P<0.05) in FFL. The n-6HUFA was highest during development larval (P<0.05). The increases DHA reflects the ability of the species to elongate and desaturate to obtain n-3HUFA from 18:3n 3, shows the importance of this fatty acid during early development.
本研究的目的是评估正交丽脂鲤亲鱼的卵母细胞、初孵仔鱼（NHL）、开口仔鱼（FFL）和肌肉组织的脂肪酸及脂质组成。在不同发育阶段的卵母细胞和仔鱼中，总脂质和极性脂质含量显著丰富（P<0.05）。肌肉中的总脂质含量最低（P<0.05），而卵母细胞、NHL和FFL的总脂质含量没有显著差异。NHL和FFL的极性脂质含量不同（P<0.05）。对于中性脂质，C18:2n 6的最低值出现在初次摄食期，而C20:4n 6（AA）在FFL中的百分比最高（P<0.05）。C22:6n 3（DHA）在FFL中含量最高（P<0.05）。雌性肌肉中的中性脂质n-9和n-6含量最高。NHL和FFL中的n-3HUFA含量最高，FFL中的n-6HUFA含量最高（P<0.05）。卵母细胞和FFL中的DHA/EPA比值较高（P<0.05）。在极性脂质的脂肪酸中，C20:5n 3（EPA）在各阶段之间没有差异（P>0.05）。C18:3n 3在NHL和FFL中含量最高（P<0.05）。C20:4n 6（AA）和C22:6n 3（DHA）在仔鱼阶段的百分比最高。FFL中的n-3系列脂肪酸显著更高（P<0.05）。发育中的仔鱼阶段n-6HUFA含量最高（P<0.05）。DHA的增加反映了该物种从18:3n 3延长和去饱和以获得n-3HUFA的能力，表明这种脂肪酸在早期发育中的重要性。
