Tocher D R, Fraser A J, Sargent J R, Gamble J C
Lipids. 1985 Feb;20(2):69-74. doi: 10.1007/BF02534210.
The fatty acid compositions of total polar and total neutral lipids of Atlantic herring eggs and larvae were determined immediately before fertilization, after fertilization and at various times during subsequent embryonic and early larval development. Within 3 hr after fertilization the percentage of total PUFA in neutral lipid decreased from 33% to 20%, with a reciprocal increase in monoenes. Thereafter the percentage of PUFA in the neutral lipids increased progressively, attaining the original level in ripe eggs by the time of yolk sac absorption. During the larval stages the percentage of PUFA continued to increase in the neutral lipid, reaching almost 44% of the total by day 32 after fertilization, although it was reduced to 32% by day 36. The percentage of monoenes in the neutral lipid displayed a progressive decrease during the whole period of development from 3 hr after fertilization. Throughout all the developmental periods the fatty acid composition of total polar lipids remained essentially constant. The polar lipids of the yolk sac displayed virtually the same fatty acid composition as the larval bodies, but the neutral lipids of the yolk sac were low in PUFA compared to the larval bodies. The results are discussed with reference to changes in lipid class composition during development. The conservation of high levels of PUFA in lipids during embryogenesis and early larval development reflects the importance of these fatty acids during development.
在受精前、受精后以及随后胚胎和幼体早期发育的不同时间，测定了大西洋鲱鱼卵和幼体中总极性脂质和总中性脂质的脂肪酸组成。受精后3小时内，中性脂质中总多不饱和脂肪酸（PUFA）的百分比从33%降至20%，单烯类则相应增加。此后，中性脂质中PUFA的百分比逐渐增加，到卵黄囊吸收时达到成熟卵中的原始水平。在幼体阶段，中性脂质中PUFA的百分比继续增加，受精后32天时达到几乎占总量的44%，不过到36天时降至32%。从受精后3小时开始的整个发育期间，中性脂质中单烯类的百分比呈逐渐下降趋势。在所有发育阶段，总极性脂质的脂肪酸组成基本保持不变。卵黄囊的极性脂质与幼体身体的脂肪酸组成几乎相同，但卵黄囊的中性脂质与幼体身体相比，PUFA含量较低。结合发育过程中脂质类别组成的变化对结果进行了讨论。在胚胎发生和幼体早期发育过程中脂质中高水平PUFA的保留反映了这些脂肪酸在发育过程中的重要性。
