Casalino M, Nicoletti M, Junakovic N, Maimone F
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Roma La Sapienza, Italy.
Antimicrob Agents Chemother. 1988 Aug;32(8):1262-6. doi: 10.1128/AAC.32.8.1262.
Class B tetracycline resistance determinants have been identified in two aerobactin-encoding FIme plasmids representative of those isolated from epidemic Salmonella wien. Genetic data, restriction enzyme analysis of recombinant and mutant plasmids, and Southern blot hybridizations indicate that in both plasmids the class B determinant so far found and described only on Tn10-like transposons is part of a different genetic element. This composite insertion sequence element is about 7 kilobases long and has copies of insertion sequence IS1 at the ends.
在从流行的维也纳沙门氏菌分离出的两种携带气杆菌素编码基因的F1me质粒中，已鉴定出B类四环素抗性决定簇。遗传数据、重组质粒和突变体质粒的限制性内切酶分析以及Southern印迹杂交表明，在这两种质粒中，迄今仅在Tn10样转座子上发现和描述的B类决定簇是不同遗传元件的一部分。这种复合插入序列元件约7千碱基长，两端有插入序列IS1的拷贝。
