Allard J D, Gibson M L, Vu L H, Nguyen T T, Bertrand K P
Department of Microbiology, Washington State University, Pullman 99164.
Mol Gen Genet. 1993 Feb;237(1-2):301-5. doi: 10.1007/BF00282811.
Plasmid pIP173, isolated from Salmonella ordonez strain BM2000, confers resistance to tetracycline and a number of other antibiotics. We determined the nucleotide sequence of the pIP173 tetR repressor and tetA resistance genes. The pIP173 tetR gene is essentially identical to the class D tetR gene from plasmid RA1. The pIP173 tet genes are flanked by directly repeated copies of the insertion sequence IS26. Interestingly, the 3' end of the tetR gene, encoding the C-terminal 16 amino acids of the TetR protein, extends into the flanking IS26 sequence. The relationships between the class A, B, C, and D TetA sequences parallel the relationships between the corresponding TetR sequences; class D is more closely related to class B than to either class A or C. Overall, the four TetA sequences show 38% identity and 57% similarity.
从奥尔顿沙门氏菌菌株BM2000中分离出的质粒pIP173赋予对四环素和许多其他抗生素的抗性。我们测定了pIP173四环素阻遏蛋白（tetR）和四环素抗性（tetA）基因的核苷酸序列。pIP173的tetR基因与质粒RA1的D类tetR基因基本相同。pIP173的tet基因两侧是插入序列IS26的直接重复拷贝。有趣的是，编码TetR蛋白C末端16个氨基酸的tetR基因的3'端延伸到侧翼的IS26序列中。A类、B类、C类和D类TetA序列之间的关系与相应TetR序列之间的关系相似；D类比A类或C类与B类的关系更密切。总体而言，这四个TetA序列显示出38%的同一性和57%的相似性。
