Sato Kuniaki, Masuda Takaaki, Hu Qingjiang, Tobo Taro, Kidogami Shinya, Ogawa Yushi, Saito Tomoko, Nambara Sho, Komatsu Hisateru, Hirata Hidenari, Sakimura Shotaro, Uchi Ryutaro, Hayashi Naoki, Iguchi Tomohiro, Eguchi Hidetoshi, Ito Shuhei, Nakagawa Takashi, Mimori Koshi
Department of Surgery, Kyushu University Beppu Hospital, Oita, Japan.
Department of Otorhinolaryngology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Anticancer Res. 2017 May;37(5):2365-2371. doi: 10.21873/anticanres.11574.
BACKGROUND/AIM: Amplification of chromosome 7p (Ch.7p) is common in colorectal cancer (CRC). The aim of this study was to identify potential driver genes on Ch.7p that are overexpressed due to DNA copy number amplification and determine their clinical significance in CRC.
We identified phosphoserine phosphatase (PSPH) as a potential driver gene using a CRC dataset from The Cancer Genome Atlas (TCGA) using a bioinformatics approach. The expression of PSPH in 124 primary CRCs was examined by quantitative reverse transcription polymerase chain reaction (PCR) and immunohistochemistry. The biological effect of PSPH expression was explored by Gene Set Enrichment Analysis (GSEA) using the TCGA dataset.
PSPH was overexpressed in tumor tissues and PSPH positively correlated with depth of invasion and distant metastasis. On multivariate analysis, high PSPH expression was an independent poor prognostic factor. These results were supported by GSEA.
PSPH could be a novel prognostic biomarker with malignant potential on Ch.7p in CRC.
背景/目的：7号染色体短臂（Ch.7p）扩增在结直肠癌（CRC）中很常见。本研究的目的是鉴定因DNA拷贝数扩增而过度表达的Ch.7p上的潜在驱动基因，并确定它们在CRC中的临床意义。
我们使用生物信息学方法，通过癌症基因组图谱（TCGA）的CRC数据集将磷酸丝氨酸磷酸酶（PSPH）鉴定为潜在驱动基因。通过定量逆转录聚合酶链反应（PCR）和免疫组织化学检测了124例原发性CRC中PSPH的表达。使用TCGA数据集通过基因集富集分析（GSEA）探索PSPH表达的生物学效应。
PSPH在肿瘤组织中过度表达，且PSPH与浸润深度和远处转移呈正相关。多因素分析显示，高PSPH表达是独立的不良预后因素。GSEA支持了这些结果。
PSPH可能是CRC中Ch.7p上具有恶性潜能的新型预后生物标志物。
