氟化钠对大鼠下颌下唾液腺细胞粘蛋白释放的刺激作用。

Stimulation of mucin release from rat submandibular salivary-gland cells by NaF.

作者信息

Shahed A R, Allmann D W

机构信息

Department of Biochemistry, Indiana University School of Medicine, Indianapolis 46223.

出版信息

Arch Oral Biol. 1988;33(5):347-51. doi: 10.1016/0003-9969(88)90068-4.

DOI:10.1016/0003-9969(88)90068-4

PMID:2847698

Abstract

The effect of NaF on cAMP accumulation, cAMP-dependent protein kinase activity (cAMP-dPK) ratios and [14C]-glucosamine-labelled mucin release from these isolated cells was investigated. NaF (0.01-5 mM) increased significantly the cellular cAMP concentration and cAMP-dPK activity ratios in a dose- and time-dependent manner. NaF (5.0 mM) increased [14C]-glucosamine-labelled mucin release in a time-dependent manner. Thus the stimulation of prelabelled mucin secretion by NaF is mediated by an increase in the cAMP concentration, which exerts its effect, at least partly, via the activation of cAMP-dPK activity.

摘要

研究了氟化钠（NaF）对这些分离细胞中环磷酸腺苷（cAMP）积累、环磷酸腺苷依赖性蛋白激酶活性（cAMP-dPK）比值以及[¹⁴C] - 氨基葡萄糖标记的粘蛋白释放的影响。NaF（0.01 - 5 mM）以剂量和时间依赖性方式显著增加细胞内cAMP浓度和cAMP-dPK活性比值。NaF（5.0 mM）以时间依赖性方式增加[¹⁴C] - 氨基葡萄糖标记的粘蛋白释放。因此，NaF对预标记粘蛋白分泌的刺激是由cAMP浓度的增加介导的，cAMP浓度的增加至少部分地通过激活cAMP-dPK活性发挥其作用。