Zhu Dan, Zhu Wenfeng, Wang Weiwei, Liu Xiaoheng
Key Laboratory for Soft Chemistry and Functional Materials of Ministry of Education, Nanjing University of Science and Technology, 200 Xiaolingwei Street, Nanjing 210094, People's Republic of China.
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
IET Nanobiotechnol. 2017 Mar;11(2):200-204. doi: 10.1049/iet-nbt.2016.0006.
Aiming to monitor hydrogen peroxide and oxidizing ions in aqueous circumstance, functionalized attapulgite [i.e. gold-modified attapulgite nanocomposites (Au/ATP NCs)] as peroxidase mimics were prepared by loading β-cysteamine-capped gold nanoparticles onto attapulgite with the use of electrostatic interactions. As-prepared Au/ATP NCs were used for the detection of HO. The linear range and detection limit for HO were determined by quantitative experiments emerging excellent stability and recyclability. The peroxidase-like activity of Au/ATP NCs could be expanded for the detection of some oxidative ions (Fe and Ag) was also been discovered. Based on the absorption spectrum and steady-state kinetics, the mechanism for peroxidase mimic reaction is investigated. This work represents the first example of multitarget detection for molecule and cations (HO, Fe and Ag) using Au/ATP NCs as peroxidase enzyme mimics and holds a promise for future biomedical and analytical applications.
为了监测水性环境中的过氧化氢和氧化离子，通过利用静电相互作用将β-巯基乙胺封端的金纳米颗粒负载到凹凸棒石上，制备了功能化凹凸棒石[即金修饰的凹凸棒石纳米复合材料（Au/ATP NCs）]作为过氧化物酶模拟物。所制备的Au/ATP NCs用于检测HO。通过定量实验确定了HO的线性范围和检测限，显示出优异的稳定性和可回收性。还发现Au/ATP NCs的过氧化物酶样活性可扩展用于检测一些氧化离子（Fe和Ag）。基于吸收光谱和稳态动力学，研究了过氧化物酶模拟反应的机制。这项工作代表了使用Au/ATP NCs作为过氧化物酶模拟物对分子和阳离子（HO、Fe和Ag）进行多目标检测的首个实例，并为未来的生物医学和分析应用带来了希望。
