Vong Minh, Ludington Jacob G, Ward Honorine D, Nibert Max L
Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, 02115, USA.
Division of Geographic Medicine and Infectious Disease, Tufts Medical Center, Boston, MA, 02116, USA.
Arch Virol. 2017 Sep;162(9):2875-2879. doi: 10.1007/s00705-017-3385-2. Epub 2017 May 5.
Bisegmented dsRNA viruses that infect most or all isolates of apicomplexan parasite Cryptosporidium parvum are currently assigned to a single species, Cryptosporidium parvum virus 1, in genus Cryspovirus, family Partitiviridae. An analysis of existing sequence data suggested that the complete sequences of both cryspovirus genome segments, dsRNA1 and dsRNA2, had yet to be determined. We therefore set out to accomplish this for the virus strain that infects C. parvum isolate Iowa. The results suggest that several previous cryspovirus sequences are indeed truncated at one or both segment termini and also identify sequences at or near the termini that are conserved in both segments. Complete sequences of other cryspovirus strains, including ones from other Cryptosporidium species, are needed for refining their classification into one or more virus species.
感染顶复门寄生虫微小隐孢子虫大多数或所有分离株的双节段双链RNA病毒，目前被归为分节段病毒科隐病毒属中的一个单一物种，即微小隐孢子虫病毒1。对现有序列数据的分析表明，隐病毒基因组片段dsRNA1和dsRNA2的完整序列尚未确定。因此，我们着手对感染微小隐孢子虫爱荷华分离株的病毒株进行此项研究。结果表明，之前的几个隐病毒序列在一个或两个片段末端确实被截断，同时还鉴定出了两个片段末端或其附近保守的序列。需要其他隐病毒株的完整序列，包括来自其他隐孢子虫物种的序列，以便将它们细化分类为一个或多个病毒物种。
