用于研究Th9细胞在癌症中作用的B16肺黑色素瘤模型

B16 Lung Melanoma Model to Study the Role of Th9 Cells in Cancer.

作者信息

Dwivedi Alka, Kumar Sushant, Purwar Rahul

机构信息

Department of Bioscience and Bioengineering, Indian Institute of Technology Bombay (IIT Bombay), Mumbai, Maharashtra, India.

出版信息

Methods Mol Biol. 2017;1585:217-222. doi: 10.1007/978-1-4939-6877-0_17.

DOI:10.1007/978-1-4939-6877-0_17

PMID:28477199

Abstract

T cell therapy has shown remarkable promise in multiple malignancies including melanoma and Acute Lymphoblastic Leukemia (ALL). Recent data demonstrated the differential efficacy of various subsets of T-helper cells in tumor regression. Th9 cells, the new member of T helper cell family, possess superior anti-tumor activity compared to Th1 and Th2 cells in murine model of melanoma. Therefore, it is important to examine the anti-tumor activity of specific subsets of Th cells in tumor models. Here, we describe the methodology of examining the anti-tumor activity of Th9 cells in murine model of melanoma.

摘要

T细胞疗法在包括黑色素瘤和急性淋巴细胞白血病（ALL）在内的多种恶性肿瘤中已显示出显著的前景。最近的数据表明，不同亚群的辅助性T细胞在肿瘤消退中具有不同的疗效。Th9细胞是辅助性T细胞家族的新成员，在黑色素瘤小鼠模型中，与Th1和Th2细胞相比，具有更强的抗肿瘤活性。因此，在肿瘤模型中研究Th细胞特定亚群的抗肿瘤活性具有重要意义。在此，我们描述了在黑色素瘤小鼠模型中检测Th9细胞抗肿瘤活性的方法。