Bueso Eduardo, Serrano Ramón, Pallás Vicente, Sánchez-Navarro Jesús A
Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia, Valencia, Spain.
Plant Physiol Biochem. 2017 Jul;116:1-8. doi: 10.1016/j.plaphy.2017.04.020. Epub 2017 Apr 21.
Seed longevity is the period during which the plant seed is able to germinate. This property is strongly influenced by environment conditions experienced by seeds during their formation and storage. In the present study we have analyzed how the biotic stress derived from the infection of Cauliflower mosaic virus (CaMV), Turnip mosaic virus (TuMV), Cucumber mosaic virus (CMV) and Alfalfa mosaic virus (AMV) affects seed tolerance to deterioration measuring germination rates after an accelerated aging treatment. Arabidopsis wild type plants infected with AMV and CMV rendered seeds with improved tolerance to deterioration when compared to the non-inoculated plants. On the other hand, CaMV infection generated seeds more sensitive to deterioration. No seeds were obtained from TuMV infected plants. Similar pattern of viral effects was observed in the double mutant athb22 athb25, which is more sensitive to accelerated seed aging than wild type. However, we observed a significant reduction of the seed germination for CMV (65% vs 55%) and healthy (50% vs 30%) plants in these mutants. The seed quality differences were overcomed using the A. thaliana athb25-1D dominant mutant, which over accumulated gibberellic acid (GA), except for TuMV which generated some siliques with low seed tolerance to deterioration. For AMV and TuMV (in athb25-1D), the seed quality correlated with the accumulation of the messengers of the gibberellin 3-oxidase family, the mucilage of the seed and the GA1. For CMV and CaMV it was not a good correlation suggesting that other factors are affecting seed viability.
种子寿命是指植物种子能够发芽的时期。这一特性受到种子在形成和储存过程中所经历的环境条件的强烈影响。在本研究中，我们分析了由花椰菜花叶病毒（CaMV）、芜菁花叶病毒（TuMV）、黄瓜花叶病毒（CMV）和苜蓿花叶病毒（AMV）感染产生的生物胁迫如何通过在加速老化处理后测量发芽率来影响种子对劣变的耐受性。与未接种的植物相比，感染AMV和CMV的拟南芥野生型植物产生的种子对劣变的耐受性有所提高。另一方面，CaMV感染产生的种子对劣变更敏感。未从感染TuMV的植物中获得种子。在双突变体athb22 athb25中观察到了类似的病毒效应模式，该双突变体比野生型对加速种子老化更敏感。然而，我们观察到这些突变体中CMV（65%对55%）和健康（50%对30%）植物的种子发芽率显著降低。使用过量积累赤霉素（GA）的拟南芥athb25-1D显性突变体克服了种子质量差异，但TuMV产生了一些种子对劣变耐受性低的角果。对于AMV和TuMV（在athb25-1D中），种子质量与赤霉素3-氧化酶家族信使、种子粘液和GA1的积累相关。对于CMV和CaMV，相关性不佳，这表明其他因素正在影响种子活力。
