北京沙尘事件期间空气中细菌的细胞浓度、活力及培养成分
Cell concentration, viability and culture composition of airborne bacteria during a dust event in Beijing.
作者信息
Yuan Hongli, Zhang Daizhou, Shi Yanning, Li Baozhen, Yang Jinshui, Yu Xuejian, Chen Nan, Kakikawa Makiko
机构信息
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, Kumamoto 862-8502, Japan.
出版信息
J Environ Sci (China). 2017 May;55:33-40. doi: 10.1016/j.jes.2016.03.033. Epub 2016 Jul 16.
Airborne bacteria were measured when a dust storm passed Beijing in spring 2012 with a focus on cell concentration, viability and TSA- and R2A-cultured strain composition. The concentration varied at an order of 10cells/m with dust loading (demonstrated with PM) and they had a very close correlation (R=0.91, p<0.01). At the time of highest PM of 652μg/m, the bacterial concentration reached 1.4×10cells/m, which was larger than that before and after the dust event by one order. Bacterial viability, the ratio of number concentration of viable cells to total cells, was 32%-64% and smaller in the dust plume than that before the dust arrival. Bacterial strains from the culture ranged between 2.5×10 and 4.6×10CFU/m and no correlation with PM was determined. Their composition was different before and after the dust arrival according to 16S rRNA gene sequences and strains belong to Actinomycetes and Firmicutes were the majority in the dust samples.
2012年春季一场沙尘暴经过北京时，对空气中的细菌进行了测量，重点关注细胞浓度、活力以及在胰蛋白胨大豆琼脂（TSA）和R2A培养基上培养的菌株组成。细菌浓度随沙尘负荷（以可吸入颗粒物PM表示）呈10倍数量级变化，二者具有非常紧密的相关性（R=0.91，p<0.01）。在可吸入颗粒物浓度最高达到652μg/m时，细菌浓度达到1.4×10⁶个细胞/m³，比沙尘事件前后高出一个数量级。细菌活力（活细胞数浓度与总细胞数的比值）为32%-64%，沙尘羽流中的细菌活力低于沙尘到来之前。培养出的细菌菌株数量在2.5×10³至4.6×10⁴CFU/m³之间，未发现与可吸入颗粒物有相关性。根据16S rRNA基因序列，沙尘到来前后细菌菌株组成不同，沙尘样本中放线菌门和厚壁菌门的菌株占多数。
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