The phosphotransferase system gene ptsI in Bacillus cereus regulates expression of sodA2 and contributes to colonization of wheat roots.

Plant growth-promoting rhizobacteria effectively enhance plant growth and root colonization by the bacteria is a prerequisite during the process. Bacillus cereus 905, a rhizosphere bacterium originally isolated from wheat roots, colonizes the wheat rhizosphere with a large population size. We previously showed that a manganese-containing superoxide dismutase (MnSOD2), encoded by the sodA2 gene, plays an important role in colonization of the wheat rhizosphere by B. cereus 905. In this study, we identified a gene, ptsI, which positively regulates transcription of sodA2. ptsI encodes Enzyme I of the phosphotransferase system (PTS), a major regulator of carbohydrate uptake in bacteria. Assays of β-galactosidase activity and real-time quantitative PCR showed that loss of ptsI caused a 70% reduction in sodA2 expression. The ΔptsI mutant also showed a 1000-fold reduction in colonization of wheat roots, as well as a reduced growth rate in minimal media with either glucose or succinate as the sole carbon source. Artificial induction of sodA2 in the ΔptsI mutant partially restored root colonizing ability and utilization of succinate, but not glucose. These results suggest that the PTS plays an important role in rhizosphere colonization by both promoting nutrient utilization and regulating sodA2 expression in B. cereus 905.