Yamasaki Eiki, Sakamoto Ryuta, Matsumoto Takashi, Maiti Biswajit, Okumura Kayo, Morimatsu Fumiki, Balakrish Nair G, Kurazono Hisao
Division of Food Hygiene, Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-11, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan.
R&D Center, NH Foods Ltd., Ibaraki, Japan.
Methods Mol Biol. 2017;1600:1-7. doi: 10.1007/978-1-4939-6958-6_1.
As cholera toxin (CT) is responsible for most of the symptoms induced by Vibrio cholerae O1 or O139 infection, detection of CT is an important biomarker for diagnosis of the disease. The procedure for pathogenicity analysis of V. cholerae isolates must be carefully developed for the reason that the amount of CT produced by V. cholerae varies according to the medium used and culture conditions (i.e. temperature and aeration status) applied. Here we describe a reproducible rapid method for analysis of CT production by toxigenic V. cholerae with an immunochromatographic test strip that can detect as low as 10 ng/mL of purified recombinant CT.
由于霍乱毒素（CT）是霍乱弧菌O1或O139感染所引发的大多数症状的致病因素，因此检测CT是该疾病诊断的一项重要生物标志物。鉴于霍乱弧菌产生的CT量会因所用培养基以及培养条件（即温度和通气状态）的不同而有所变化，所以必须谨慎制定霍乱弧菌分离株的致病性分析程序。在此，我们描述了一种可重现的快速方法，用于通过免疫层析试纸条分析产毒霍乱弧菌的CT产生情况，该试纸条能够检测低至10 ng/mL的纯化重组CT。
