Hanselaar A G, Vooijs G P, Oud P S, Pahlplatz M M, Beck J L
Department of Pathology, University of Nijmegen, The Netherlands.
Cancer. 1988 Dec 15;62(12):2537-45. doi: 10.1002/1097-0142(19881215)62:12<2537::aid-cncr2820621215>3.0.co;2-w.
This study presents the results of cytophotometric (CPM) and flow cytometric (FCM) DNA ploidy measurements in cervical intraepithelial neoplasias grade III (CIN III) with and without synchronous invasive squamous cell carcinoma. Hysterectomy and biopsy material from 21 patients 35 years of age or younger and from 18 patients age 50 years or older was studied. The DNA analysis was performed in nuclei isolated from specific areas of paraffin-embedded tissue. There were significant differences in the distribution of DNA patterns between the two age groups. About 80% of CIN III lesions in women 50 years of age or older, with or without a coexisting invasive cancer were aneuploid. In the group of younger women a diploid DNA pattern was found in about 60% of CIN III with concomitant invasive cancer. In the absence of an invasive cancer, CIN III lesions were mostly polyploid. The DNA pattern of invasive cancers was generally identical with the adjacent CIN, thus suggesting that the two lesions were related. Although the prognostic value of DNA ploidy measurements in cervical intraepithelial lesions in women in these two age groups has to be further evaluated, these results are at considerable variance with previously published data on DNA values in CIN and invasive carcinoma. In four CIN III lesions without invasive cancer, in women of the group of 35 years of age or younger, human papilloma virus common antigen could be demonstrated by immunochemical procedure. In three of these cases a polyploid DNA pattern was present; the fourth case showed a bimodal aneuploid pattern.
本研究呈现了对伴有和不伴有同步浸润性鳞状细胞癌的宫颈上皮内瘤变III级（CIN III）进行细胞光度测定（CPM）和流式细胞术（FCM）DNA倍性测量的结果。对21例35岁及以下患者和18例50岁及以上患者的子宫切除标本和活检材料进行了研究。DNA分析在从石蜡包埋组织的特定区域分离出的细胞核中进行。两个年龄组之间的DNA模式分布存在显著差异。在50岁及以上的女性中，无论是否存在并存的浸润性癌，约80%的CIN III病变为非整倍体。在较年轻女性组中，约60%伴有浸润性癌的CIN III呈现二倍体DNA模式。在无浸润性癌的情况下，CIN III病变大多为多倍体。浸润性癌的DNA模式通常与相邻的CIN相同，因此表明这两种病变相关。尽管这两个年龄组女性宫颈上皮内病变中DNA倍性测量的预后价值有待进一步评估，但这些结果与先前发表的关于CIN和浸润性癌DNA值的数据有很大差异。在4例无浸润性癌的35岁及以下女性的CIN III病变中，通过免疫化学方法可检测到人乳头瘤病毒共同抗原。其中3例呈现多倍体DNA模式；第4例显示双峰非整倍体模式。
