Hanselaar A G, Vooijs G P, Pahlplatz M M
Department of Pathology, University Hospital Nijmegen, The Netherlands.
Cytometry. 1990;11(5):624-9. doi: 10.1002/cyto.990110510.
Cervical intraepithelial neoplasia grade III (CIN III) and squamous cell carcinoma (INV) were examined using DNA ploidy and cytophotometric analysis. Based on hysterectomy, exconisation, and biopsy material from 69 patients in two age categories, analysis was performed in nuclei isolated from selected areas of paraffin-embedded tissue. High percentages of DNA-diploidy in INV lesions were found mainly in the group of patients age 45 years or younger. CIN III lesions in women age 46 or older demonstrated high percentages of DNA-aneuploidy. DNA-polyploidy was most frequent in CIN III lesions in the younger age category. The results of cytophotometric analysis indicated that the overall mean values of 16 nuclear photometric features discriminated significantly between the whole groups of CIN III (n = 37) and INV (n = 32). On an individual patient level, however, the mean feature values showed a large overlap. Based on the results of a stepwise linear discriminant analysis of patient mean values, a combination of geometrical and run-length texture features was used to discriminate between CIN III and INV lesions. The correct classification rate was highest in the category of patients in the older age category. The results of this study indicate age related differences in CIN III and invasive squamous cell carcinoma, and they may be of help in assessing cytophotometric features in the study of progressive and non-progressive CIN lesions.
采用DNA倍体分析和细胞光度分析对宫颈上皮内瘤变Ⅲ级（CINⅢ）和鳞状细胞癌（INV）进行检测。基于来自两个年龄组69例患者的子宫切除术、锥切术和活检材料，对石蜡包埋组织选定区域分离出的细胞核进行分析。在INV病变中，高比例的DNA二倍体主要见于45岁及以下的患者组。46岁及以上女性的CINⅢ病变显示出高比例的DNA非整倍体。DNA多倍体在较年轻年龄组的CINⅢ病变中最为常见。细胞光度分析结果表明，16个细胞核光度特征的总体平均值在CINⅢ（n = 37）和INV（n = 32）的整个组之间有显著差异。然而，在个体患者水平上，平均特征值显示出很大的重叠。基于患者平均值的逐步线性判别分析结果，采用几何特征和游程纹理特征的组合来区分CINⅢ和INV病变。年龄较大组患者的正确分类率最高。本研究结果表明CINⅢ和浸润性鳞状细胞癌存在年龄相关差异，它们可能有助于在进行性和非进行性CIN病变研究中评估细胞光度特征。
