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5'上游序列和串联启动子在rpsU-dnaG-rpoD大分子合成操纵子调控中的作用。

Role of the 5' upstream sequence and tandem promoters in regulation of the rpsU-dnaG-rpoD macromolecular synthesis operon.

作者信息

Nesin M, Lupski J R, Godson G N

机构信息

Biochemistry Department, New York University Medical Center, New York 10016.

出版信息

J Bacteriol. 1988 Dec;170(12):5759-64. doi: 10.1128/jb.170.12.5759-5764.1988.

DOI:10.1128/jb.170.12.5759-5764.1988
PMID:2848013
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211679/
Abstract

Bal31 exonuclease deletion analysis and transposon Tn5 mutagenesis of the 5' regulatory region of the rpsU-dnaG-rpoD macromolecular synthesis operon fused to the chloramphenicol acetyltransferase gene (pGLR301) demonstrated that sequences 5' to the operon promoters were not involved in operon transcriptional regulation and that the three tandem promoters P1, P2, and P3 were functionally independent. P2 was the strongest promoter, and P3 was the weakest. P1, P2, and P3 acting in combination appeared to be stronger than the individual promoters.

摘要

Bal31核酸外切酶缺失分析以及与氯霉素乙酰转移酶基因（pGLR301）融合的rpsU-dnaG-rpoD大分子合成操纵子5'调控区的转座子Tn5诱变表明，操纵子启动子5'端的序列不参与操纵子的转录调控，并且三个串联启动子P1、P2和P3在功能上是独立的。P2是最强的启动子，P3是最弱的。P1、P2和P3共同作用似乎比单个启动子更强。

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J Bacteriol. 1988 Dec;170(12):5759-64. doi: 10.1128/jb.170.12.5759-5764.1988.
2
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本文引用的文献

1
Identification of ribosomal protein S7 as a repressor of translation within the str operon of E. coli.鉴定核糖体蛋白S7为大肠杆菌str操纵子内翻译的阻遏物。
Cell. 1981 May;24(2):413-9. doi: 10.1016/0092-8674(81)90331-7.
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Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
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The rpsU-dnaG-rpoD macromolecular synthesis operon of E. coli.大肠杆菌的rpsU-dnaG-rpoD大分子合成操纵子。
Cell. 1984 Dec;39(2 Pt 1):251-2. doi: 10.1016/0092-8674(84)90001-1.
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Cell. 1984 Sep;38(2):371-81. doi: 10.1016/0092-8674(84)90492-6.
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The nucleotide sequence of an Escherichia coli operon containing genes for the tRNA(m1G)methyltransferase, the ribosomal proteins S16 and L19 and a 21-K polypeptide.一个大肠杆菌操纵子的核苷酸序列，该操纵子包含tRNA（m1G）甲基转移酶、核糖体蛋白S16和L19以及一种21K多肽的基因。
EMBO J. 1983;2(6):899-905. doi: 10.1002/j.1460-2075.1983.tb01519.x.
6
Compilation and analysis of Escherichia coli promoter DNA sequences.大肠杆菌启动子DNA序列的汇编与分析
Nucleic Acids Res. 1983 Apr 25;11(8):2237-55. doi: 10.1093/nar/11.8.2237.
7
Plasmid vectors for the selection of promoters.用于启动子选择的质粒载体。
Gene. 1984 Feb;27(2):151-60. doi: 10.1016/0378-1119(84)90136-7.
8
The use of transposon Tn5 mutagenesis in the rapid generation of correlated physical and genetic maps of DNA segments cloned into multicopy plasmids--a review.转座子Tn5诱变技术在快速构建克隆于多拷贝质粒的DNA片段的相关物理图谱和遗传图谱中的应用——综述
Gene. 1984 Feb;27(2):131-49. doi: 10.1016/0378-1119(84)90135-5.
9
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.pUC质粒，一种源自M13mp7的用于插入诱变和使用合成通用引物进行测序的系统。
Gene. 1982 Oct;19(3):259-68. doi: 10.1016/0378-1119(82)90015-4.
10
Cloning and characterization of the Escherichia coli chromosomal region surrounding the dnaG Gene, with a correlated physical and genetic map of dnaG generated via transposon Tn5 mutagenesis.大肠杆菌dnaG基因周围染色体区域的克隆与特性分析，以及通过转座子Tn5诱变产生的dnaG相关物理图谱和遗传图谱。
Mol Gen Genet. 1982;185(1):120-8. doi: 10.1007/BF00333800.