5'上游序列和串联启动子在rpsU-dnaG-rpoD大分子合成操纵子调控中的作用。
Role of the 5' upstream sequence and tandem promoters in regulation of the rpsU-dnaG-rpoD macromolecular synthesis operon.
作者信息
Nesin M, Lupski J R, Godson G N
机构信息
Biochemistry Department, New York University Medical Center, New York 10016.
出版信息
J Bacteriol. 1988 Dec;170(12):5759-64. doi: 10.1128/jb.170.12.5759-5764.1988.
Abstract
Bal31 exonuclease deletion analysis and transposon Tn5 mutagenesis of the 5' regulatory region of the rpsU-dnaG-rpoD macromolecular synthesis operon fused to the chloramphenicol acetyltransferase gene (pGLR301) demonstrated that sequences 5' to the operon promoters were not involved in operon transcriptional regulation and that the three tandem promoters P1, P2, and P3 were functionally independent. P2 was the strongest promoter, and P3 was the weakest. P1, P2, and P3 acting in combination appeared to be stronger than the individual promoters.
摘要
Bal31核酸外切酶缺失分析以及与氯霉素乙酰转移酶基因（pGLR301）融合的rpsU-dnaG-rpoD大分子合成操纵子5'调控区的转座子Tn5诱变表明，操纵子启动子5'端的序列不参与操纵子的转录调控，并且三个串联启动子P1、P2和P3在功能上是独立的。P2是最强的启动子，P3是最弱的。P1、P2和P3共同作用似乎比单个启动子更强。
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