通过对rpsU-dnaG-rpoD大分子合成操纵子上游5kb大肠杆菌染色体区域进行分子分析所揭示的潜在新基因。

Possible new genes as revealed by molecular analysis of a 5-kb Escherichia coli chromosomal region 5' to the rpsU-dnaG-rpoD macromolecular-synthesis operon.

作者信息

Nesin M, Lupski J R, Svec P, Godson G N

出版信息

Gene. 1987;51(2-3):149-61. doi: 10.1016/0378-1119(87)90303-9.

Abstract

The complete nucleotide sequence of 5-kb DNA fragment immediately 5' to the rpsU-dnaG-rpoD macromolecular-synthesis operon in Escherichia coli has been determined. It encodes for six open reading frames. Transcriptional and translational analysis have shown that three of them (orfx, orfz1 and orfz2) are expressed in exponentially growing E. coli cells. The orfx, directly 5' to the rpsU gene but transcribed in the opposite direction, may be part of the rpsU-dnaG-rpoD macromolecular-synthesis operon.

摘要

已确定大肠杆菌中紧邻rpsU-dnaG-rpoD大分子合成操纵子5'端的一段5kb DNA片段的完整核苷酸序列。它编码六个开放阅读框。转录和翻译分析表明，其中三个（orfx、orfz1和orfz2）在指数生长的大肠杆菌细胞中表达。orfx直接位于rpsU基因的5'端，但转录方向相反，可能是rpsU-dnaG-rpoD大分子合成操纵子的一部分。